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SYRAP_pDONR201

Plasmid

#249814

Purpose

Encodes SYRAP (RAP2.12-GAL4STE12), Gateway compatible as Donor plasmid

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Insert

RAP2.12-GAL4STE (RAP2.12 Budding Yeast, Mustard Weed)

Use

Synthetic Biology

Tags

GAL4-STE12

Expression

Plant and Yeast

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79b(P3-Myc-nTEV-IRES-EBFP)

Plasmid

#248357

Purpose

Lentiviral vector that can express P3 along with N terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P3-Myc-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato)

Plasmid

#248356

Purpose

Lentiviral vector that can express P4 along with C terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P4-GGGSGGG-cTEV-HA

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1D

Plasmid

#183340

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1D

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1C

Plasmid

#183339

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1C

Use

Lentiviral

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-Y11

Plasmid

#239000

Purpose

Final circuit with -Ara: ON, +Ara: OFF behaviour

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

Circuit with sfGFP expression only in the absence of Ara (-Ara: ON, +Ara: OFF)

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pANC76(TVMVp-IRES-dTomato)

Plasmid

#248362

Purpose

Lentiviral vector that can express TVMVp. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

TVMVp

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3717

Plasmid

#240491

Purpose

CRISPRi-mediated Knockdown of RPA3717

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA3701

Plasmid

#240490

Purpose

CRISPRi-mediated Knockdown of RPA3701

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSSBIO32_dCas9_RPA1580

Plasmid

#240488

Purpose

CRISPRi-mediated Knockdown of RPA1580

Depositor

Rajib Saha

Article

Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

Insert

Cas9 (cas9 Streptococcus pyogenes)

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Plac

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pNTA-uTEV3-GSG-t2A-EBFP

Plasmid

#248360

Purpose

Lentiviral vector that can express uTEV3. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

uTEV3-GSG-t2A-EBFP

Use

Lentiviral

Promoter

tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pANC75(HCV NS3p-IRES-dTomato)

Plasmid

#248361

Purpose

Lentiviral vector that can express HCV NS3p. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

HCV NS3p

Use

Lentiviral

Promoter

tight TRE

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_BRD4

Plasmid

#183331

Purpose

All-in-One CRISPRko system with a guide RNA that targets BRD4 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

BRD4

Use

Lentiviral

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(H114F)

Plasmid

#245204

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

H114F

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(F29K)

Plasmid

#245222

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

F29K

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(C104P)

Plasmid

#245202

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

C104P

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(T507K)

Plasmid

#245213

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

T507K

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(D106P)

Plasmid

#245203

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

D106P

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(I282V)

Plasmid

#245210

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

I282V

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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