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PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B[T22N]

Plasmid

#24909

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

fishRab8B

Use

Gateway entry vector

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pRB7-6

Plasmid

#24700

Depositor

George Allen

Article

Allen et al Plant Cell. 1996 May . 8(5):899-913.

Insert

Tobacco Rb7 MAR (SAR)

Use

E. coli phagemid

Available Since

July 8, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO V5 SSA1

Plasmid

#19516

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Tags

V5

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 DNAK

Plasmid

#19517

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Tags

V5

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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PA1024_pET21a(+)C-tag

Plasmid

#14579

Depositor

SeWon Suh

Article

Ha et al J Biol Chem. 2006 Jul 7. 281(27):18660-7

Insert

2-nitropropane dioxygenase (PA1024 Pseudomonas aeruginosa)

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHL_R1_EGFP

Plasmid

#250888

Purpose

EGFP expressed under PHL synthetic promoter ; part of BNeu AS8; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHL

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHT_R4_EGFP

Plasmid

#250889

Purpose

EGFP expressed under PHT synthetic promoter and regulated by RBS 'R4'; part of BNeuAS9; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PHT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and C55I

Plasmid

#248807

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and C55I

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-mCherry-Flag-LplAW37V and R337P

Plasmid

#248805

Purpose

Constructs used for LplA-catalyzed labeling in mammalian cells. LplA is expressed as a fusion with mCherry.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-mCherry-Flag

Expression

Mammalian

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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His6-TEV-LplA W37V and R337P

Plasmid

#248817

Purpose

Plasmid used for bacterial expression of LplA variants. TEV cleavage site enables removal of N-terminal His6 tag.

Depositor

Alice Ting

Article

Cavanagh et al Science. 2026 Jan 8:eadv7953. doi: 10.11

Insert

LplA

Tags

6xHis-TEV

Expression

Bacterial

Mutation

W37V and R337P

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pHK101

Plasmid

#235480

Purpose

Repression of gene VIII by Ptet promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII, cI repressor

Expression

Bacterial

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pHK408

Plasmid

#235458

Purpose

AND gate receiver with bs for sgRNA2 and sgRNA3

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

AND gate

Use

Synthetic Biology

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1580_ECOR31HamAB_CoPurification

Plasmid

#223371

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1370_pTet-ECOR31_Hachiman

Plasmid

#223364

Purpose

Expression of ECOR31 Hachiman under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1465_pTet-ECOR31Ham_DELhamB_Mut

Plasmid

#223366

Purpose

Expression of ECOR31 Hachiman HamA (∆HamB) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamA (∆HamB)

Expression

Bacterial

Mutation

∆HamB

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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