We narrowed to 5,641 results for: pcas

1. pEX-PK-hLC3

   Plasmid

   #61458

   Purpose

   Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy

   Depositor

   Isei Tanida

   Article

   Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

   Insert

   PK-hLC3 (MAP1LC3B Human, Fission Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH-ABE8e-spG

   Plasmid

   #200775

   Purpose

   For plant adenine base editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

   Insert

   nCas9-spG(D10A)-TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

   Promoter

   maize Ubiquitin-1, OsU3

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. R2pH-LAMPx3ALFA

   Plasmid

   #225136

   Purpose

   R2pH-LAMP1-labelled lysosomes with 3xLFA

   Depositor

   Massimiliano Stagi

   Article

   Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

   Insert

   Lamp1 (Lamp1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. kiCAP-AAV-BI-hTFR1-2

   Plasmid

   #218797

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGB 35s:dCas:BRD:tNos (GB1172)

   Plasmid

   #75401

   Purpose

   Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   dCas9:BRD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE7.10-F148A

   Plasmid

   #132946

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   ABE7.10(F148A)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   ABE7.10(F148A)

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-COX8A

    Plasmid

    #227308

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of COX8A (COX8A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U67

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-U6-sgGAA

    Plasmid

    #232724

    Purpose

    Cas9 guide RNA targeting GAA repeats for A-to-G base editing

    Depositor

    David Liu

    Article

    Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

    Insert

    SpCas9 gRNA targeting GAA repeats
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. TadA-NG-CBE

    Plasmid

    #227420

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEX-PK-hLC3∆G

    Plasmid

    #61459

    Purpose

    Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy

    Depositor

    Isei Tanida

    Article

    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

    Insert

    PK-hLC3∆G (MAP1LC3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. BLBP-mCherry

    Plasmid

    #63721

    Purpose

    Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

    Depositor

    Bart De Strooper

    Article

    Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

    Insert

    BLBP (Fabp7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1700 bp upstream of BLBP gene cloned in CAG-mCher…

    Promoter

    1700bp upstream of BLBP gene

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid

    #104571

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    

    Use

    CRISPR

    Tags

    NLS-3xHA-3xFLAG-4xDmrA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-PE

    Plasmid

    #162796

    Purpose

    All-in-one prime editor piggyBac transposon

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_T7-PE2-Nuclease

    Plasmid

    #171997

    Purpose

    Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CMV_T7-Cas9-RT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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