We narrowed to 23,437 results for: c-myc
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Plasmid#135814PurposeFluorescent reporter for knock-in of TdTomato into the TH locusDepositorInsertTH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
Tagsp2a-TdTomatoExpressionMammalianAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G12V
Plasmid#111850PurposeExpresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G12V (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 12 to ValinePromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APPP1-mGFP
Plasmid#196705PurposeAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmCherry and mEGFPExpressionMammalianMutationLys to Val substitution at position 612Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro
Plasmid#25949DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α bHLH* P405A/P531A Δ820-870-pBabe-Puro
Plasmid#25957DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceSept. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APPP1-mGFP
Plasmid#196695PurposeAs mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmEGFP and mtagBFP2ExpressionMammalianMutationLys to Val substitution at position 612PromoterCMVAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpRY
Plasmid#179082PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variantDepositorInsertSpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…PromoterCAGAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001146057)
Plasmid#76376Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148320)
Plasmid#76378Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AP-APP
Plasmid#196706PurposeExpresses APP695 with a biotin Acceptor Peptide tag at N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsAP, Avitag and HAExpressionMammalianAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV-NanoLuc-BRAF-CAT
Plasmid#194772PurposeMammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.DepositorInsertBRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
TagsNanoLucExpressionMammalianPromoterCMV51Available SinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148673)
Plasmid#76377Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1 gRNA (BRDN0001147582)
Plasmid#77732Purpose3rd generation lentiviral gRNA plasmid targeting human ABL1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV mAG beta-catenin
Plasmid#206258PurposeENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.DepositorInsertmAG Ctnnb1 (Ctnnb1 Mouse)
UseMultimate/gateway entr 1Tagsmonomeric Azami GreenExpressionMammalianAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX-53BP1(1-1710) 3LA
Plasmid#185698PurposeMammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.DepositorInsert53BP1(1-1710) 3LA (TP53BP1 Human)
UseRetroviralTagsFlag tag, HA tag, and tetracysteine tagExpressionMammalianMutationL175A, L517A, and L693APromoterSV40Available SinceJuly 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMX-53BP1(1-1710) 3LA7A
Plasmid#185707PurposeMammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.DepositorInsert53BP1(1-1710) 3LA7A (TP53BP1 Human)
UseRetroviralTagsFlag tag, HA tag, and tetracysteine tagExpressionMammalianMutationT302A, S452A, S523A, S543A, S625A, S784A, and S89…PromoterSV40Available SinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENT-L1-Kozak-EGFP-GPI-stop-L2
Plasmid#186355PurposeEntry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequencesDepositorInsertsignal peptide of human CD59, eGFP, aa 67-102 of human CD59 which contains the GPI attachment site (CD59 Human, Synthetic)
UseExpression of a fluorescent membrane markerTagsEGFPAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ERBB3 gRNA (BRDN0001147088)
Plasmid#77481Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB3DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABL1 gRNA (BRDN0001145303)
Plasmid#77733Purpose3rd generation lentiviral gRNA plasmid targeting human ABL1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only