We narrowed to 10,617 results for: t7

1. pRSET-cfp29

   Plasmid

   #233651

   Purpose

   Mycobacterium tuberculosis Encapsulin CFP29 expression plasmid in E. coli

   Depositor

   Ye Gao

   Article

   Berger et al Commun Biol. 2025 Feb 15;8(1):245. doi:

   Insert

   Encapsulin
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINK-RYBP NZF (20-51)

   Plasmid

   #235652

   Purpose

   Bacterial expression of human RYBP NZF (20-51)

   Depositor

   David Komander

   Article

   Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

   Insert

   RYBP NZF (RYBP Human)
   

   Tags

   6xHis-GST-3c

   Expression

   Bacterial

   Mutation

   aa 20-51 only

   Promoter

   T7

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINS-HOIP NZF1 (351-379)

   Plasmid

   #235655

   Purpose

   Bacterial expression of human HOIP NZF1 (351-379)

   Depositor

   David Komander

   Article

   Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

   Insert

   HOIP NZF1 (RNF31 Human)
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   aa 351-379 only

   Promoter

   T7

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINK-ZRANB3 NZF (619-650)

   Plasmid

   #235654

   Purpose

   Bacterial expression of human ZRANB3 NZF (619-650)

   Depositor

   David Komander

   Article

   Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

   Insert

   ZRANB3 NZF (ZRANB3 Human)
   

   Tags

   6xHis-GST-3c

   Expression

   Bacterial

   Mutation

   aa 619-650 only

   Promoter

   T7

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINK-Sharpin NZF (347-376)

   Plasmid

   #235651

   Purpose

   Bacterial expression of human Sharpin NZF (347-376)

   Depositor

   David Komander

   Article

   Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

   Insert

   Sharpin NZF (SHARPIN Human)
   

   Tags

   6xHis-GST-3c

   Expression

   Bacterial

   Mutation

   aa 347-376 only

   Promoter

   T7

   Available Since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. MZB164

   Plasmid

   #217842

   Purpose

   10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expression

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   HaloTag
   

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_Gcn4 solvvol W

   Plasmid

   #231878

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest17_Gcn4 solvvol

   Plasmid

   #231877

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest17_Gcn4 SATtoG KtoR

   Plasmid

   #231861

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   T7

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINF HopF2

    Plasmid

    #228397

    Purpose

    Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopF2 (hopF2 Pseudomonas syringae pv. tomato)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-AviTag-CC-S2

    Plasmid

    #233243

    Purpose

    Bacterial expression of Avi-CC-S2

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    AviTag-CC-S2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Kif3A-EGFP-SNAP

    Plasmid

    #229852

    Purpose

    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments

    Depositor

    William Hancock

    Insert

    Kif3A (Kif3a Mouse)
    

    Tags

    6xHis, EGFP, and SNAP-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLANT-2b

    Plasmid

    #231487

    Purpose

    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.

    Depositor

    Venkat Gopalan

    Inserts

    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    

    Tags

    His6 tag and His6-SUMO^NIT

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…

    Promoter

    T7 promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcRII-mogat3a in situ probe

    Plasmid

    #232135

    Purpose

    zebrafish mogat3a anti-sense in situ probe, linearize with NotI, SP6 polymerase

    Depositor

    Steven Farber

    Article

    Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

    Insert

    mogat3a
    

    Use

    In vitro transcription

    Promoter

    SP6/T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. polySUMO3

    Plasmid

    #221067

    Purpose

    Expression of polySUMO3 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Halo-BASU-His

    Plasmid

    #218451

    Purpose

    E coli expression vector for Halo-BASU-His biotin ligase

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    HaloTag-BASU-His fusion protein
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. MZB216

    Plasmid

    #221766

    Purpose

    "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    rtxA
    

    Promoter

    T7 Promoter

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. E547* FL USP7

    Plasmid

    #229001

    Purpose

    E547-mutant of human USP7

    Depositor

    Irina Bezsonova

    Insert

    USP7 1-546 (USP7 Human)
    

    Tags

    6xHis cleavable with thrombin

    Expression

    Bacterial

    Mutation

    E547 mutated to stop codon

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSF-G1(CysCNP)RS

    Plasmid

    #225054

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Chem 102385

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVF53

    Plasmid

    #229477

    Purpose

    expresses RssBE229C

    Depositor

    Alexandra Deaconescu

    Article

    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

    Insert

    RssB (E229C)
    

    Expression

    Bacterial

    Mutation

    E229C

    Promoter

    T7

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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