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AiP1349 - pAAV-AiE2E120m-minBGpromoter-SYFP2-WPRE3-bGHpA

Plasmid

#208137

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus. Alias: AiP1349 - pAAV-AiE2120m-minBG-SYFP2-WPRE-BGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AiP1346 - pAAV-AiE2E117m-minBGpromoter-SYFP2-WPRE3-bGHpA

Plasmid

#208135

Purpose

Direct-expressing SYFP2 in astrocytes AAV Virus. Alias: AiP1346 - pAAV-AiE2117m-minBG-SYFP2-WPRE-BGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

Beta Globin minimal promoter

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFPnls barcode-210-SV40 polyA

Plasmid

#190876

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFPnls-barcode210

Use

AAV

Mutation

WT

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK

Plasmid

#202541

Purpose

An adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoter

Depositor

Christopher Richie

Article

Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

Insert

Gas6 (Gas6 Mouse)

Use

AAV

Tags

Myc-DDK

Mutation

Deletion of F50-E275

Promoter

SYN1

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/CBA::EGFP-P2A-tC3/54aa-FLAG

Plasmid

#129479

Purpose

AAV2 transfer plasmid for truncated C3 (54 aa) tagged with FLAG with EGFP under control of the CBA promoter

Depositor

Robert Gross

Insert

truncated (AA148-201) exoenzyme C3 from C. botulinum, P2A, EGFP

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

Plasmid

#188539

Purpose

An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.

Depositor

Brandon Harvey

Insert

secreted EGFP

Use

AAV

Tags

CDNF(1-28) and THPKTEL WPRE

Promoter

CMV-IE

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine

Plasmid

#186419

Purpose

Expression of shRNA for the knock down of mouse Egr3

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

miREgr3-5D

Use

AAV

Tags

mCitrine

Promoter

human Synapsin1

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

Plasmid

#185717

Purpose

AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

Depositor

Jun Ding

Article

Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

Insert

synuclein, alpha (SNCA Human)

Use

AAV

Mutation

Changed Ala 11 to Pro, Val 70 to Pro

Promoter

hSyn

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

Plasmid

#153195

Purpose

encodes pAAV NovArch

Depositor

Adam Cohen

Article

Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

Insert

NovArch

Use

AAV

Tags

citrine

Expression

Mammalian

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1049-pAAV-mscRE13-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163482

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1049 - pAAV-AiE2013m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP319-pAAV-U6SaCas9gRNA(emx1sg2)-EFS-GFP-KASH-pA

Plasmid

#113696

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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1476_pAAV-U6-SA-mMttp-gRNA-N21-HLP-SACas9-spA

Plasmid

#109315

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a gRNA against Mttp

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

Mttp gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIA4-2xmiR-1 target sites

Plasmid

#120299

Purpose

AAV vector for expression of AcrIIA4 with two miR-1 binding sites

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIA4-2xmiR-1 binding sites

Use

AAV and CRISPR

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

Plasmid

#120300

Purpose

AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC1

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP318-pAAV-U6SaCas9gRNA(emx1sg1)-EFS-GFP-KASH-pA

Plasmid

#113695

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

Plasmid

#114373

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv4.2LL (NEWENTRY )

Use

AAV

Tags

EYFP and Kv4.2LL

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

Plasmid

#114375

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C (NEWENTRY )

Use

AAV

Tags

EYFP and Kv2.1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

Plasmid

#114372

Purpose

expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Nlgn1C (NEWENTRY )

Use

AAV

Tags

EYFP and Nlgn1C

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

Plasmid

#60228

Purpose

Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA

Expression

Mammalian

Promoter

CBh and U6

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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