We narrowed to 170,988 results for: addgene

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pYSD_lambda_mRFP

Plasmid

#217831

Purpose

Yeast surface display Fab vector with lambda light chain and mRFP

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_lambda

Plasmid

#217829

Purpose

Mini-mutagenesis plasmid for lambda light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMMP_kappa

Plasmid

#217828

Purpose

Mini-mutagenesis plasmid for kappa light chain YSD Fabs

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Type

Empty backbone

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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3xMS2 TR knockin sgRNA 2

Plasmid

#207608

Purpose

sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

acccccaaacctgactgact

Tags

None

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN155(I152L)-SRCR5

Plasmid

#207993

Purpose

BiFC assay

Depositor

Young Tang

Article

Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

Insert

SRCR5 (CD163 pig)

Expression

Mammalian

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209016

Purpose

Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[K5A]-FLAG

Plasmid

#199326

Purpose

mutated ubiquitination site (ubiquitination inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 5 to Alanine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[K5R]-FLAG

Plasmid

#199325

Purpose

mutated ubiquitination site (ubiquitination active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 5 to Arginine

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-CSE1L neo

Plasmid

#192341

Purpose

Lentiviral expression vector for an inducible 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

CSE1L (CSE1L Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-DYNLL2 zeo

Plasmid

#192321

Purpose

Lentiviral expression vector for an inducible 3xFLAG-DYNLL2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

DYNLL2 (DYNLL2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-BCAS2

Plasmid

#192294

Purpose

Gateway entry vector for an inducible 3xFLAG-BCAS2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

BCAS2 (BCAS2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/CaMKIIa::iLMO2

Plasmid

#129481

Purpose

AAV2 transfer plasmid for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical, under control of the glutamatergic-selective CaMKIIa promoter

Depositor

Robert Gross

Article

Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

Insert

iLMO2

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.031

Plasmid

#184968

Purpose

Test effect of extending -Eco2 a1/a2 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(extended), a1/a2 length: 29

Expression

Yeast

Mutation

human codon optimized RT, a1/a2 length extended t…

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.108

Plasmid

#184977

Purpose

Express -Eco1 LYP1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12

Expression

Yeast

Mutation

LYP1 donor E27stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.106

Plasmid

#184975

Purpose

Express -Eco1 CAN1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12

Expression

Yeast

Mutation

CAN1 donor G444stop

Promoter

Gal7

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.72

Plasmid

#184984

Purpose

Express Cas9/-Eco1 RT fusion, using linker1

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker1-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.95

Plasmid

#184986

Purpose

Express Cas9/-Eco1 RT fusion, using linker2

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Cas9-fusion_linker2-Eco1RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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