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pJS-BCD-Strep-dPCaspase

Plasmid

#214035

Purpose

Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Tags

Strep-tag II

Expression

Bacterial

Mutation

H79A, C146A

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCaspase-R153A

Plasmid

#214036

Purpose

Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCaspase

Tags

Strep-tag II

Expression

Bacterial

Mutation

R153A

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCX-TRI-2A

Plasmid

#74674

Purpose

mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Inserts

HO1 (HMOX1 Human)
CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRV-GN

Plasmid

#106373

Purpose

creating stable line with GFP and G418 resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

eGFP and geneticin/G418 resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12C)_BRAF-NanoLuc

Plasmid

#238566

Purpose

Express HaloTag-KRAS(G12C)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12C

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12D)_BRAF-NanoLuc

Plasmid

#238563

Purpose

Express HaloTag-KRAS(G12D)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12V)_BRAF-NanoLuc

Plasmid

#238565

Purpose

Express HaloTag-KRAS(G12V)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PCDH-WT-ALKBH5-3xFLAG

Plasmid

#203736

Purpose

Overexpression of ALKBH5-WT

Depositor

Jianjun Chen

Article

Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

Insert

ALKBH5 (ALKBH5 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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TRCN0000297275

Plasmid

#78152

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pF CAG luc-EGFP-cre puro

Plasmid

#67503

Purpose

Constitutive expression of a firefly luciferase-enhanced green fluorescent protein-cre recombinase fusion protein in mammalian cells

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

luciferase-EGFP-cre

Use

Cre/Lox, Lentiviral, and Luciferase ; Fluorescent…

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Kras (G12V)-pcw107

Plasmid

#64602

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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PCDH-Mut-ALKBH5-3xFLAG

Plasmid

#203737

Purpose

Overexpression of ALKBH5-Mut

Depositor

Jianjun Chen

Article

Shen et al Cell Stem Cell. 2020 Jul 2;27(1):64-80.e

Insert

ALKBH5 (ALKBH5 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Enzyme Inactive Mutation (H226A)

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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TRCN0000338462

Plasmid

#78158

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000338401

Plasmid

#78157

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000338399

Plasmid

#78156

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pLEX307-mFzd8

Plasmid

#102870

Purpose

Expresses mouse Fzd8 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 8 (Fzd8 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

T to C substitution at nucleotide 1134, C to T su…

Promoter

EF1A

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Rgl2-CAAX-pcw107-V5

Plasmid

#64641

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Rgl2 plus C-term KRAS (Kras Mouse)

Use

Lentiviral

Tags

V5

Mutation

C-term of Kras

Promoter

PGK

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only
TRCN0000279887

Plasmid

#78153

Purpose

shCDK4 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000279952

Plasmid

#78155

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

Lentiviral and RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only