We narrowed to 11,587 results for: Kars

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nuclear EKAR (EGFP-mRFP)

Plasmid

#18682

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Tags

EGFP and mRFP

Expression

Mammalian

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-blast

Plasmid

#167818

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-smURFP-StagRFP-EKAR-EV

Plasmid

#173019

Purpose

Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

smURFP-StagRFP-EKAR-EV

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN5-ires-blast

Plasmid

#167822

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-msGFP2-HDEL

Plasmid

#160468

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signal

Depositor

Benjamin Glick

Article

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-msGFP2-HDEL

Expression

Yeast

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMDC32-Kar2-CFP-HDEL

Plasmid

#96989

Purpose

Express endoplasmic reticulum (ER) marker in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

Kar2-CFP-HDEL

Expression

Plant

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-FLIM-AKART391A

Plasmid

#60446

Purpose

Expresses the non-phosphorylatable point mutant (T391A) of FLIM-AKAR in a Cre dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKART391A

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

changed Threonine 391 to Ananine

Promoter

CAG

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-blast

Plasmid

#167829

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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