We narrowed to 1,590 results for: PTS

1. FU- dio Teal-gephyrin - W

   Plasmid

   #73918

   Purpose

   To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

   Depositor

   Elly Nedivi

   Article

   Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

   Insert

   Teal-gephyrin
   

   Use

   Lentiviral

   Tags

   Teal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.10/RSV_SF3B3-Xon_Luciferase

   Plasmid

   #174659

   Purpose

   Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   Xon cassette
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-p150Glued

   Plasmid

   #36154

   Depositor

   David Stephens

   Article

   Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

   Insert

   p150Glued (DCTN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   see documentation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLC133

   Plasmid

   #198639

   Purpose

   Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

   Depositor

   David Bikard

   Article

   Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

   Insert

   dCas9
   

   Use

   Tags

   3x-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. HeLa full-length MLCK-GFP

   Plasmid

   #46316

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase (MYLK Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human SERCA3 (pMT2)

   Plasmid

   #75189

   Purpose

   Mammalian expression of ATP2A3

   Depositor

   Jonathan Lytton, David MacLennan

   Article

   Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

   Insert

   ATP2A3 (ATP2A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   adenovirus major late promoter

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hela kinase-dead MLCK-GFP

   Plasmid

   #46317

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase-dead (MYLK Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   E1626K

   Promoter

   CMV

   Available since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pIRES2-EGFP Rac2

   Plasmid

   #12193

   Depositor

   Gary Johnson

   Article

   Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

   Insert

   Rac2 (RAC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEYFP-Sec24D

   Plasmid

   #66614

   Purpose

   YFP fusion of Sec24D

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

   Insert

   Sec24D (SEC24D Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

10. GFP-Myo 1c

    Plasmid

    #134832

    Purpose

    Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal GFP-tag.

    Depositor

    Martin Bähler

    Article

    Ruppert et al J Cell Sci. 1995 Dec;108 ( Pt 12):3775-8

    Insert

    Myo 1c (rat) with 3' UTR (Myo1c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid

    #176531

    Purpose

    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

    Depositor

    Jared Toettcher

    Article

    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

    Inserts

    KGV transcription factor
    TagBFP
    

    Use

    Piggybac transposase integration vector

    Tags

    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Histone H2B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45216

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    Histone H2B-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Rix-PGK-Tom-W

    Plasmid

    #25813

    Depositor

    Patrick Salmon, Jozsef Kiss

    Article

    Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

    Insert

    Rix-PGK-Tom-W
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    tdTomato from Tsien lab

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    t7

    Available since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSin wPGK-Cre

    Plasmid

    #101242

    Purpose

    low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    Cre recombinase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    see attached file

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-SRGAP1FBAR(516a.a.)-EGFP

    Plasmid

    #168505

    Purpose

    Expression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.

    Depositor

    Franck Polleux

    Article

    Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

    Insert

    SRGAP1-FBAR (SRGAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1-516

    Promoter

    CAG

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-ING1b

    Plasmid

    #79052

    Purpose

    expression of p33ING1b in mammalian cells

    Depositor

    Karl Riabowol

    Article

    Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

    Insert

    ING1 (p33 ING1Ab isoform) (ING1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5 FRT TO myc hSeparase

    Plasmid

    #59820

    Purpose

    Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1α-mouse-retroCHMP3-HA

    Plasmid

    #154178

    Purpose

    expresses mouse retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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