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pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

Plasmid

#180012

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-NRasQ61K

Plasmid

#89886

Purpose

Human NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Publication

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-NRasQ61K

Use

Unspecified

Tags

mCherry

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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p060 pCS mTRPS1

Plasmid

#11032

Depositor

Ramesh Shivdasani

Publication

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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MGCO-27 tol2-IsceI-eef1al1:mStayGold:sec61b-SV40-attB-IsceI-tol2

Plasmid

#258401

Purpose

mStayGold reporter for sec61b in zebrafish

Depositor

Gokul Upadhyayula

Insert

sec61b (SEC61B Human)

Use

Zebrafish expression

Tags

mStayGold

Promoter

eef1a1l1

Available since

Aug. 28, 2026

Availability

Academic Institutions and Nonprofits only

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60-p3E_INTRON-EmptyRNAi

Plasmid

#163380

Purpose

p3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).

Depositor

Jean Giacomotto

Type

Empty backbone

Use

RNAi

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194518

Purpose

ZSP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Publication

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Publication

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP

Plasmid

#73200

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Publication

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

CFP

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-FRT-GalK-cryaa-YFP

Plasmid

#73198

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Publication

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

YFP

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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M28 Nac>GFP

Plasmid

#17178

Depositor

Randall Moon

Publication

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter (mitfa Zebrafish)

Tags

GFP

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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acta1-mRuby2-VHH[GFP]

Plasmid

#163856

Purpose

Zebrafish muscle specific expression of mRuby2-VHH[GFP]. Parton lab clone FRX

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

VHH[GFP]

Use

In vitro transcription of rna

Tags

Ruby2

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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ZE7 Pax2

Plasmid

#17056

Depositor

Randall Moon

Publication

Kelly et al Dev Genet. 1995. 17(2):129-40.

Insert

pax b (pax2a Zebrafish)

Use

Zebrafish expression

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Publication

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T178V, S203A, S207A, S212A

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pGCS-N3(3xFLAG)

Plasmid

#85720

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Publication

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGCS-N2(3xHA)

Plasmid

#85719

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Publication

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Publication

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

Plasmid

#174026

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-QF2

Use

Zebrafish knock-in tagging

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFT1.1

Plasmid

#46139

Purpose

Tol2-based FlExTrap vector. mCherry is in the "+1" reading frame starting from the exon.

Depositor

Wenbiao Chen

Publication

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

mCherry

Use

Cre/Lox; Mutagenic gene trap for zebrafish

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-YFP-Smo

Plasmid

#31618

Depositor

Rajat Rohatgi

Publication

Milenkovic et al J Cell Biol. 2009 Nov 2. 187(3):365-74.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Smo (Smo Mouse)

Use

Zebrafish, avian expression

Tags

YFP

Expression

Mammalian and Worm

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pME-Zebrabow

Plasmid

#226792

Purpose

Middle-entry vector with Zebrabow cassette. For use in Gateway cloning.

Depositor

Florence Marlow

Publication

Pahima et al Development. 2026 Jul 6:dev.204904. doi:

Insert

Zebrabow

Use

Gateway cloning middle-entry vector

Available since

July 28, 2026

Availability

Academic Institutions and Nonprofits only

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