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We narrowed to 5,049 results for: golden gate

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Showing: 1301 - 1350 of 5049 results

  1. pKS1576

    Plasmid
    #239524
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239524-plasmid-map-sequence-id-475895

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  2. C80m

    Plasmid
    #120992
    Purpose
    MoClo golden gate assembly CD part for mfLon protease (see doi: [10.1038/nbt.3053]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120992-plasmid-map-sequence-id-236095

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  3. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid
    #126881
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126881-plasmid-map-sequence-id-249630

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  4. C86m

    Plasmid
    #120997
    Purpose
    MoClo golden gate assembly CD part (phage lysis protein; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    X174E
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120997-plasmid-map-sequence-id-236126

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  5. C11m

    Plasmid
    #120959
    Purpose
    MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Citrine
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120959-plasmid-map-sequence-id-238041

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  6. C5m

    Plasmid
    #120957
    Purpose
    MoClo golden gate assembly CD part for mRFP (monomeric red fluorescent protein; see https://doi.org/10.1073/pnas.082243699 and https://www.fpbase.org/protein/mrfp1/).
    Depositor
    Richard Murray
    Insert
    mRFP
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120957-plasmid-map-sequence-id-236154

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  7. 35S:AMBERv2 (pICSL11381)

    Plasmid
    #252522
    Purpose
    Level 1 plasmid in second position containing 35S:AMBERv2 (pICSL11381)
    Depositor
    Sophien Kamoun
    Insert
    AMBERv2
    Expression
    Plant
    Promoter
    Short 35s + Ω
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252522-plasmid-map-sequence-id-508748

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  8. 35S:GOLD (pICSL11383)

    Plasmid
    #252523
    Purpose
    Level 1 plasmid in second position containing 35S:GOLD (pICSL11383)
    Depositor
    Sophien Kamoun
    Insert
    GOLD
    Expression
    Plant
    Promoter
    Short 35s + Ω
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    252523-plasmid-map-sequence-id-506599

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  9. pSIC(P0)

    Plasmid
    #251364
    Purpose
    Backbone for construct integration in Synechocystis locus slr1311 for CyanoConstruct system.
    Depositor
    Kate Adamala, Pauli Kallio
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    251364-plasmid-map-sequence-id-503724

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  10. pDFC(P0)

    Plasmid
    #251363
    Purpose
    Replicative expression plasmid (shuttle vector) backbone for CyanoConstruct system.
    Depositor
    Kate Adamala, Pauli Kallio
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    251363-plasmid-map-sequence-id-505319

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  11. 35S:AMBERv1 (pICSL11382)

    Plasmid
    #252521
    Purpose
    Level 1 plasmid in second position containing 35S:AMBERv1 (pICSL11382)
    Depositor
    Sophien Kamoun
    Insert
    AMBERv1
    Expression
    Plant
    Promoter
    Short 35s + Ω
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252521-plasmid-map-sequence-id-504945

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  12. 35S:RUBY (pICSL11384)

    Plasmid
    #252524
    Purpose
    Level 1 plasmid in second position containing 35S:RUBY (pICSL11384)
    Depositor
    Sophien Kamoun
    Insert
    RUBY
    Expression
    Plant
    Promoter
    Short 35s + Ω
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252524-plasmid-map-sequence-id-504944

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  13. (P3)syfp2

    Plasmid
    #251374
    Purpose
    Gene encoding yellow fluorescent protein variant
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    syfp2
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251374-plasmid-map-sequence-id-503700

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  14. pL0M-SC-RUBY

    Plasmid
    #245485
    Purpose
    Golden Gate Level 0 "SC" RUBY module
    Depositor
    Beth Rowan
    Insert
    RUBY
    Expression
    Plant
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    245485-plasmid-map-sequence-id-488120

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  15. pKS1338

    Plasmid
    #239511
    Purpose
    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    AtUTR1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Nov. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239511-plasmid-map-sequence-id-489601

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  16. pKS1337

    Plasmid
    #239512
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Nov. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239512-plasmid-map-sequence-id-489589

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  17. pKS1335

    Plasmid
    #239509
    Purpose
    Express Ymd8p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYmd8p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239509-plasmid-map-sequence-id-491723

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  18. pKS1336

    Plasmid
    #239510
    Purpose
    Express Hut1p membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScHut1p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    239510-plasmid-map-sequence-id-487900

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  19. pKS1517

    Plasmid
    #239518
    Purpose
    Express ClPT1(37-407) membrane enzyme cargo and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-ClPT1(37-407)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239518-plasmid-map-sequence-id-475888

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  20. pKS1518

    Plasmid
    #239519
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239519-plasmid-map-sequence-id-475880

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  21. pKS1135

    Plasmid
    #239497
    Purpose
    Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2
    Depositor
    John Dueber
    Insert
    Ost1ss-sfCherry3C(11)-Pex3_TMD(+/-6AA)-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239497-plasmid-map-sequence-id-475868

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  22. pAIP4_2H7W

    Plasmid
    #234220
    Purpose
    Heterologous protein expression of chagasin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234220-plasmid-map-sequence-id-464185

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  23. pAIP4_2NT3

    Plasmid
    #234208
    Purpose
    Heterologous protein expression of response regulator homolog in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2NT3
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234208-plasmid-map-sequence-id-464173

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  24. U22m

    Plasmid
    #121025
    Purpose
    MoClo golden gate assembly BC part (high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD6
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121025-plasmid-map-sequence-id-245900

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  25. C32m

    Plasmid
    #120967
    Purpose
    MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb CinR
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120967-plasmid-map-sequence-id-236380

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  26. C36m

    Plasmid
    #120970
    Purpose
    MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase for 3OC14). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohCD carb RhlI
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120970-plasmid-map-sequence-id-245423

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  27. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  28. C59m

    Plasmid
    #120976
    Purpose
    MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mScarlet
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120976-plasmid-map-sequence-id-236030

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  29. C13m

    Plasmid
    #120960
    Purpose
    MoClo golden gate assembly CD part for Cerulean (modified CFP; variant of https://www.fpbase.org/protein/cerulean/) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Cerulean
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120960-plasmid-map-sequence-id-245422

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  30. P1m

    Plasmid
    #120934
    Purpose
    MoClo golden gate assembly AB part plasmid for J23101 (strength 1791x Anderson promoter; see http://parts.igem.org/Promoters/Catalog/Anderson); AB overhangs.
    Depositor
    Richard Murray
    Insert
    J23101
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120934-plasmid-map-sequence-id-235942

    Enlarge

  31. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid
    #126882
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126882-plasmid-map-sequence-id-249649

    Enlarge

  32. pAIP4_5Y33

    Plasmid
    #234204
    Purpose
    Heterologous protein expression of alginate lyase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5Y33
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234204-plasmid-map-sequence-id-464169

    Enlarge

  33. P18m

    Plasmid
    #120941
    Purpose
    MoClo golden gate assembly AB part for T7 promoter (strong, T7 polymerase-dependent constitutive promoter; use with C95m). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    T7
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120941-plasmid-map-sequence-id-235947

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  34. C31m

    Plasmid
    #120966
    Purpose
    MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    bxb1 integrase
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120966-plasmid-map-sequence-id-236888

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  35. P9m

    Plasmid
    #120937
    Purpose
    MoClo golden gate assembly AB part for pOR1-OR2 (lambda cI-repressable strong promoter) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Pr-OR1-OR2
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120937-plasmid-map-sequence-id-247706

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  36. (P2)RBSS7

    Plasmid
    #251372
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251372-plasmid-map-sequence-id-503741

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  37. (P6)RBSS6

    Plasmid
    #251391
    Purpose
    Synthetic RBS used in expression in Synechocystis
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S6
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251391-plasmid-map-sequence-id-503746

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  38. (P6)RBSS5

    Plasmid
    #251390
    Purpose
    RBS from rbcL gene; slr0090 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S5
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251390-plasmid-map-sequence-id-503745

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  39. (P6)RBSS7

    Plasmid
    #251392
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251392-plasmid-map-sequence-id-503744

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  40. (P2)RBSS6

    Plasmid
    #251371
    Purpose
    Synthetic RBS used in expression in Synechocystis
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S6
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251371-plasmid-map-sequence-id-503743

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  41. (P2)RBSS5

    Plasmid
    #251370
    Purpose
    RBS from rbcL gene; slr0090 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S5
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251370-plasmid-map-sequence-id-503727

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  42. (P4)RBSS6

    Plasmid
    #251381
    Purpose
    Synthetic RBS used in expression in Synechocystis
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S6
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251381-plasmid-map-sequence-id-503715

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  43. (P4)RBSS7

    Plasmid
    #251382
    Purpose
    RBS originally used in pDF-lac [74]
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S7
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251382-plasmid-map-sequence-id-503712

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  44. (P4)RBSS5

    Plasmid
    #251380
    Purpose
    RBS from rbcL gene; slr0090 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S5
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251380-plasmid-map-sequence-id-503680

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  45. pNDMS291

    Plasmid
    #248098
    Purpose
    sfCFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248098-plasmid-map-sequence-id-495377

    Enlarge

  46. pNDMS290

    Plasmid
    #248097
    Purpose
    sfYFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248097-plasmid-map-sequence-id-495376

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  47. pNDMS237

    Plasmid
    #248095
    Purpose
    sfYFP fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248095-plasmid-map-sequence-id-495374

    Enlarge

  48. pNDMS238

    Plasmid
    #248094
    Purpose
    sfCFP fluorescent reporter for synthetic Mesorhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Meso_PsnifH
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248094-plasmid-map-sequence-id-495373

    Enlarge

  49. pKS1614

    Plasmid
    #239545
    Purpose
    Express truncated VoGES, Idi1, and Erg20WW for synthesis of geraniol in the peroxisome with Venus as peroxisomal marker
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239545-plasmid-map-sequence-id-479032

    Enlarge

  50. pKS1429

    Plasmid
    #239514
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239514-plasmid-map-sequence-id-478375

    Enlarge

Showing: 1301 - 1350 of 5049 results