We narrowed to 24,865 results for: Nes
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Plasmid#221446PurposeMammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
TagsNuclear Export Signal (NES) and eGFPExpressionMammalianMutationNES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…PromoterCMVAvailable SinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP
Plasmid#221444PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
Tags3XFlag and Nuclear Export Signal (NES)ExpressionMammalianMutationNES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …PromoterCMVAvailable SinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP
Plasmid#194697PurposeHuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_on-mEGFP
UseZebrafish expressionTagsmEGFPPromoterelavl3(HuC)Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP
Plasmid#194698PurposeHuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_off-mEGFP
UseZebrafish expressionTagsmEGFPPromoterelavl3(HuC)Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP
Plasmid#205704PurposeGal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila fliesDepositorInsertCaprola5-EGFP
ExpressionInsectPromoter20 Gal4Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-4493NES(Booster-PKA)
Plasmid#184711PurposePKA biosensorDepositorInsertBooster-PKA
ExpressionMammalianAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-mCBP80
Plasmid#192313PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-mCBP80DepositorInsertmCh-V5-NES-EGFP-mCBP80
UseGateway entry vectorTags3xFLAGAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT mCh-V5-NES-EGFP-IBB
Plasmid#192314PurposeGateway entry vector for an inducible mCh-V5-NES-EGFP-IBBDepositorInsertmCh-V5-NES-EGFP-IBB
UseGateway entry vectorTags3xFLAGAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-myc-OGA(GS-544)C181_NES
Plasmid#193998Purposeplasmid encoding myc-tagged OGA(GS-544)C181_NESDepositorInsertmyc-OGA(GS-544)C181_NES
TagsmycExpressionMammalianAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS
Plasmid#107303PurposeExpresses ZFP-VEGFA-TS2 fused to FRB in mammalian cellsDepositorInsertZFP_VEGFA-TS2
UseCRISPRTags2xNES, 3x Flag, 2xNLS, and FRBExpressionMammalianPromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS
Plasmid#107304PurposeExpresses ZFP-VEGFA-TS3 fused to FRB in mammalian cellsDepositorInsertZFP_VEGFA-TS3
UseCRISPRTags2xNES, 3x Flag, 2xNLS, and FRBExpressionMammalianPromoterCMV IE94Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub-RLuc8N-NES
Plasmid#138213PurposeEncodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES
Plasmid#138299PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub(T/A)-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)PromoterEF1aAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-NES-jRGECO1a-WPRE
Plasmid#216276PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1aDepositorInsertNES-jRGECO1a
UseAAV and Cre/LoxTags6XHIS and XpressPromoterhuman synapsin 1 (hSyn)Available SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
TagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP
Plasmid#173029PurposeFor expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.DepositorInsertpspCas13b
UseCRISPR and LentiviralTags3xFLAG, BFP, HIV NES, and T2AExpressionMammalianPromoterEf1aAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-2xV5-TurboID-NES-STOP_IDG-K
Plasmid#158162PurposeGateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsert2xV5-TurboID-NES
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#101061PurposeAAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2-F391W
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES
Plasmid#196828PurposeExpress dpdpCas13b with AAAA mutationDepositorInsertdpspCas13b(AAAA)
Tags3XFLAG and NESExpressionMammalianMutationK367A, D369A and K370AAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only