-
Plasmid#153332PurposeTriggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertHA4-7c12 Tandem Monobody
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP92-TD-HilA
Plasmid#153331PurposeTriggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinoseDepositorInsertSptP92-HA4-7c12 Tandem Monobody
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCASP-3G124-HilA
Plasmid#153324PurposeShows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vectorDepositorInsert3G124 DARPin but no expression
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
myc-Miro2 (lacking TM)
Plasmid#127614PurposeHuman Miro2 with the transmembrane deletedDepositorInsertMiro2 (RHOT2 Human)
UseTagsmycExpressionMammalianMutationTransmembrane deletionPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SPATA13-1/1
Plasmid#91458PurposeProtein expression and purification of human SH3 domain construct SPATA13-1/1DepositorInsertSPATA13-1/1 (SPATA13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
myc-AP4 Epsilon
Plasmid#127623PurposeN-terminal myc-tagged AP4 epsilon subunitDepositorInsertAP-4 complex subunit epsilon-1 (AP4E1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NQO1-Flag
Plasmid#61729PurposeExpresses human NQO1 Flag tagged at C-terminusDepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTagsFlagExpressionMammalianMutationPromotercmvAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mRuby2
Plasmid#40260PurposeRFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertmRuby2
UseTagsHis6 and XpressExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3-Clover
Plasmid#40259PurposeGFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlapDepositorHas ServiceCloning Grade DNAInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceSept. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-TEV-NQO1
Plasmid#119058PurposeFor expression in E.coli and affinity purification of N-terminally His tagged NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTags6His, TEV siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pWPI_SPbFGF
Plasmid#25812DepositorInsertpWPI_SPbFGF (FGF2 Human)
UseLentiviralTagsExpressionMutationIg signal peptide in N-terminal of human FGF2PromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Yap1 Y357F
Plasmid#18882DepositorInsertYap1 Y357F (YAP1 Human)
UseTagsFlagExpressionMammalianMutationY357FPromoterAvailable sinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCW17 - pAdapter
Plasmid#174664PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.DepositorInsertMS2 coat protein
UseTagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
CYFIP2.mCherry
Plasmid#122052PurposeExpresses human CYFIP2 in mammalian cells.DepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35u4
Plasmid#174786PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid systemDepositorInsertMS2 coat protein
UseTagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 S156A
Plasmid#127588PurposePhospho-mutant of S156DepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutationS156APromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Miro1 S156E
Plasmid#127589PurposePhospho-mimetic of S156DepositorInsertMiro1 (RHOT1 Human)
UseTagseGFPExpressionMammalianMutationS156EPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only