We narrowed to 2,200 results for: Xpa

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pEC2861 (pSpy1C-PgyrA_ffluc)

Plasmid

#218511

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PgyrA

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRS42H_PTEF1_dCas9_TCYC1

Plasmid

#177155

Purpose

Expresses dCas9 in yeast cells

Depositor

Gautam Dantas

Article

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Insert

Nuclease-null Cas9

Tags

SV40 NLS

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-7m1-QFBDADM1

Plasmid

#46097

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-RUNX2+10A-IDR

Plasmid

#145251

Purpose

Expresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2A+10A-IDR

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pQLL-Mariner2_AG

Plasmid

#217063

Purpose

Donor plasmid of Mariner2_AG Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Mariner2_AG transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1883

Plasmid

#154331

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1849

Plasmid

#154327

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pEvoltac-EcW-TGA-h14

Plasmid

#218764

Purpose

encodes EcWRS-h14 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h14

Expression

Bacterial

Mutation

S8A, V144G, V146C

Promoter

tacI

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBM19-sd4U

Plasmid

#179692

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE4-DddA-G1397-C (T1413I)

Expression

Bacterial

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBEAST2-sfGFP

Plasmid

#126575

Purpose

Strong constitutive expression of super-folder GFP for cell-free expression

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

sfGFP

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMK485 (3mAID-Hygro)

Plasmid

#214397

Purpose

3mAID for N-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

3mAID-Hygro

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK448 (BromoTag-BSD)

Plasmid

#214378

Purpose

BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

BromoTag-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-C (LEU2)

Plasmid

#177796

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[3]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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RCASBP(A)-3xFlag-mHoxd13+7Ala

Plasmid

#145289

Purpose

Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(+7Ala) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13(+7A)-IDR-mCherry

Plasmid

#145281

Purpose

Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Expression

Bacterial

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMK466 (mAB-BSD)

Plasmid

#214392

Purpose

mAID-BromoTag for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

mAID-BromoTag-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ230-STU

Plasmid

#138110

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

LbCas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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