We narrowed to 18,335 results for: met

1. pALS2-sfGFP 150TAG

   Plasmid

   #197574

   Purpose

   Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcBac1-Ma-sfGFP 150TAG

   Plasmid

   #197568

   Purpose

   Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   M. alvus Pyl-tRNA (6) (4x copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mApple-M1 Spastin

   Plasmid

   #134461

   Purpose

   M1 Spastin expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met to Ala change at amino acid 87

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. hHS1-M7A

   Plasmid

   #159170

   Purpose

   Human codon-optimized cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   hHS1-M7A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Mutated Met 7 of the cyt b562 module of hHS1 to A…

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. yHS1-M7A

   Plasmid

   #159151

   Purpose

   Cytosolic labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   yHS1-M7A
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of yHS1 to A…

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pBa-flag-BicD2 594-FKBP

   Plasmid

   #64206

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Use

   Tags

   FKBP and flag

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mito-yHS1-M7A

   Plasmid

   #159164

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A
   

   Use

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of mito-yHS1…

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pMXs-Etv3

   Plasmid

   #131610

   Purpose

   expression of mouse Etv3 in mammalian cells

   Depositor

   Kateri Moore, Filipe Pereira

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Etv3 (Etv3 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. nuc-yHS1-M7A

   Plasmid

   #159167

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1-M7A
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid

    #101347

    Purpose

    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    delta Tat

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP(1-158)-STOP

    Plasmid

    #66107

    Purpose

    This vector attaches YFP(1-158) to the C-terminus of a protein

    Depositor

    Catherine Berlot

    Article

    Hu et al J Biol Chem. 2013 Apr 19;288(16):11416-3

    Insert

    YFP(1-158)STOP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Gln69 was changed to Met.

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)

    Plasmid

    #61943

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Residues 2-39 deleted resulting in construct star…

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

13. HA tagged mid ELL2

    Plasmid

    #127274

    Purpose

    Expresses human ELL2 with HA tag in the middle that does not disrupt function

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 with HA at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HA inserted at XbaI after Met 186

    Promoter

    pEF1a

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid

    #197566

    Purpose

    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    N166A, V168G, W239C

    Promoter

    CMV and U6/H1

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPos-Ma-CmR-TAG111

    Plasmid

    #197571

    Purpose

    Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Chloramphenicol resistance gene - 111TAG
    T7 polymerase -TAG
    GFPuv
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    111TAG and 8TAG/112TAG

    Promoter

    AraC, T7, cat promoter (constitutive), and lpp (c…

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. M3-M14-dCas9

    Plasmid

    #134779

    Purpose

    Expression of fusion protein M3-M14-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Promoter

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pComb3HSS

    Plasmid

    #64133

    Purpose

    2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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