We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1_ZKSCAN1_Tau10-12K317M

   Plasmid

   #194164

   Purpose

   Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau K317M mutation. Expresses the tau circRNA 12-->10 K317M with 3X flag tag in exon 10.

   Depositor

   Stefan Stamm

   Article

   Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

   Insert

   Microtubule-associated protein tau 10-12 WT (MAPT Human)
   

   Use

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Lysine 317 to Methionine

   Promoter

   Available since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MUC2 D1

   Plasmid

   #181812

   Purpose

   Expresses human MUC2 D1, residues 21-389

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc2 D1 (MUC2 Human)
   

   Use

   Tags

   6xHistadine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-R/G-ICUE

   Plasmid

   #181849

   Purpose

   ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   R/G-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-rsAKARev(T/A)

   Plasmid

   #166232

   Purpose

   Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

   Depositor

   Peter Dedecker

   Article

   Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

   Insert

   Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 506 in rsAKARev to Alanine

   Promoter

   CMV

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-V5-SPR

   Plasmid

   #127236

   Purpose

   Eukaryotic expression of SPR

   Depositor

   Kai Johnsson

   Article

   Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

   Insert

   SPR (SPR Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_SNORD2 probe

   Plasmid

   #73070

   Purpose

   antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD2 (SNORD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1_SNORD60 probe

   Plasmid

   #73071

   Purpose

   antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD60 (SNORD60 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-VEGFR1-V5/HIS

   Plasmid

   #205616

   Purpose

   Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR1 receptor tyrosine kinase (FLT1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-VEGFR2-V5/HIS

   Plasmid

   #205617

   Purpose

   Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR2 receptor tyrosine kinase (KDR Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Neo(+) hSUMF1

    Plasmid

    #24386

    Depositor

    Carolyn Bertozzi

    Article

    Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

    Insert

    hSUMF1 (SUMF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-EGFR-V5/HIS

    Plasmid

    #201102

    Purpose

    expression of human EGFR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid

    #205618

    Purpose

    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    T28M, S455R- Please see depositor comment

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_nV5-AMOT (p130)

    Plasmid

    #172997

    Purpose

    constitutive expression of V5-tagged AMOT (p130)

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOT (p130) (AMOT Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Flag-NPM1wt

    Plasmid

    #131810

    Purpose

    Expresses Flag-NPM1wildtype (human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-GGamma9-GFP2

    Plasmid

    #140991

    Purpose

    Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma9-GFP2 (GNGT2 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-OTUB1 WT-pcDNA3.1

    Plasmid

    #118209

    Purpose

    Expresses FLAG-OTUB1 WT in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1.Gαq-LgB97

    Plasmid

    #134360

    Purpose

    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαq-LgB97 (GNAQ Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MET-V5/HIS

    Plasmid

    #201982

    Purpose

    expression of human MET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MET receptor tyrosine kinase (MET Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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