We narrowed to 7,530 results for: Ski;

1. pESF140_HisSpy_DDB1∆B

   Plasmid

   #124213

   Purpose

   Insect cell expression vector for His6, Spy tagged hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Tags

   His6-Spy

   Expression

   Insect

   Mutation

   internal deletion of BPB domain to aid crystalliz…

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6_HEK3+1T>A_(PP7-C4-Q1)

   Plasmid

   #232437

   Purpose

   pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_HEK3_nsgRNA(PP7)

   Plasmid

   #232444

   Purpose

   nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY1623 NOLC1 sgRNA 2

   Plasmid

   #234834

   Purpose

   sgRNA 2 for NOLC1 STITCHR insertion

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

   Insert

   NOLC1 sgRNA (NOLC1 Human)
   

   Use

   CRISPR

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-sgSLC7A11/xCT-2

   Plasmid

   #161819

   Purpose

   knock out SLC7A11/xCT in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC

   Plasmid

   #229545

   Purpose

   Lentiviral construct to express rtTA and SKM proteins

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   rTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hEF1a

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-CXCR4

   Plasmid

   #98946

   Purpose

   Mammalian expression plasmid for N-terminal myc-tagged human CXCR4

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Tags

   Signal/leader sequence from Influenza A virus HA …

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid

    #73499

    Purpose

    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDY1622 NOLC1 sgRNA 1

    Plasmid

    #234833

    Purpose

    sgRNA 1 for NOLC1 STITCHR insertion

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    NOLC1 sgRNA (NOLC1 Human)
    

    Use

    CRISPR

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQCXIZ GFP PODXL A WT

    Plasmid

    #202413

    Purpose

    Expression of GFP-tagged PODXL (isoform A) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIZ GFP PODXL B WT

    Plasmid

    #202414

    Purpose

    Expression of GFP-tagged PODXL (isoform B) WT

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-C2m2-mKate

    Plasmid

    #208793

    Purpose

    For AAV production to express C2m2-mKate in astrocytes

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    AAV

    Tags

    mKate

    Expression

    Mammalian

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    GFAP promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-PEmax SpG

    Plasmid

    #187895

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Grb2 SH2-SH3

    Plasmid

    #46442

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb2 (GRB2 Human)
    

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 1-217

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-puro-N-3HA-SREBP1-C-Flag

    Plasmid

    #134286

    Purpose

    Lentivector encoding 3XHA (N-term) and Flag (C-term)-tagged SREBP1

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP1 (Srebf1 Mouse)
    

    Use

    Lentiviral

    Tags

    3x HA and Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP1 isoform a precursor

    Promoter

    EF1a

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid

    #191097

    Purpose

    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-vsfGFP-0
    

    Use

    AAV

    Tags

    Green fluorescent protein bound to enhancer nanob…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDY0750 pCAG-NLS-BceINT

    Plasmid

    #179112

    Purpose

    BceINT expression vector

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-BceINT
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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