We narrowed to 11,977 results for: pcDNA

1. pcDNA-Prom1 SV8 (-Ex19a)

   Plasmid

   #184012

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701).

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 SV8(-Ex19a) (Prom1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of exon 19a (amino acids 696-701).

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA T7-Gnat1 Mut-3UTR

   Plasmid

   #184027

   Purpose

   Expresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGA

   Depositor

   Peter Stoilov

   Article

   Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

   Insert

   Gnat1 (Gnat1 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   TAG sites in the 3'-UTR are mutated to TGA

   Promoter

   CMV

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Hygro(+)_GRHL1-R9A-L3F6H

   Plasmid

   #177767

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (R9A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R9A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_Hygro(+)_GRHL1-S95A-L3F6H

   Plasmid

   #177768

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S95A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S95A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

   Plasmid

   #177769

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K116A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

   Plasmid

   #177770

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T139A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

   Plasmid

   #177771

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K370A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

   Plasmid

   #177772

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S374A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

   Plasmid

   #177773

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K592A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-MS2-HA-HsSmg5-G120E_W

    Plasmid

    #147819

    Purpose

    Mammalian Expression of HsSmg5-G120E

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg5-G120E (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

    Plasmid

    #147558

    Purpose

    Mammalian Expression of HsSmg7_633-1091

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsSmg7_633-1091 (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation V854I compared to the seq…

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-myc-TRBP-dsRBD2

    Plasmid

    #180602

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-myc-TRBP-dsRBD1

    Plasmid

    #180601

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-α-COPI-WD40-Arg57Ala

    Plasmid

    #180759

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Arg57Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Arg57Ala, Leu1…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid

    #180760

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Asp115Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Asp115Ala, Leu…

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-TRBP-L2+D2+L3-myc/His

    Plasmid

    #180600

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Flag-gp130dimer-stm2585

    Plasmid

    #174395

    Purpose

    Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain and sarA chimera
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Flag-stm2585-gp130

    Plasmid

    #174393

    Purpose

    Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    140-177 of sarA replaced with homologous IL6ST se…

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3HA-AMOT p130∆PPxY2

    Plasmid

    #166712

    Purpose

    expresses AMOT with inactivating mutation in second PPxY motif in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 P262A P263A (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed proline 262 to alanine, proline 263 to al…

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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