Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 11,261 results for: 158

Clear keywords
Showing: 5401 - 5420 of 11261 results

  2. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

    Enlarge

  4. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid
    #114080
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114080-plasmid-map-sequence-id-230482

    Enlarge

  8. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

    Plasmid
    #114091
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R and S542R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114091-plasmid-map-sequence-id-220538

    Enlarge

  16. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

    Enlarge

  17. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid
    #114079
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114079-plasmid-map-sequence-id-231032

    Enlarge

  18. pTU1-A-gap_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107577
    Purpose
    B. megaterium DSM319 gap promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    gapdh promoter - B. megaterium DSM319
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107577-plasmid-map-sequence-id-209152

    Enlarge

Showing: 5401 - 5420 of 11261 results