Addgene

Showing: 5401 - 5420 of 15204 results

Results per page:

Sort results by:

pCDNA3-Flag-Tpr

Plasmid

#60882

Purpose

Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

Depositor

Vinay Nandicoori

Article

Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

Insert

Tpr (TPR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-p38beta

Plasmid

#86833

Purpose

mammalian expression of GFP-p38beta

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38beta (MAPK11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGPTVII-SED1

Plasmid

#232615

Purpose

SED1 biosensor for expression in Arabidopsis thaliana

Depositor

Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 Biosensor

Use

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-mTagBFP2-2A-Puro_hU6-RfxDR36-BsmBI

Plasmid

#226011

Purpose

CasRx guide RNA cloning backbone (lenti)

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_NOPlight1

Plasmid

#195578

Purpose

Expresses NOPLight1 in mammalian cells

Depositor

Tommaso Patriarchi

Article

Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

Insert

NOPLight1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

Plasmid

#223175

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Tags

Expression

Mutation

DR1 A>T

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP/golgin-160

Plasmid

#180630

Purpose

Expression of WT golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160 (GOLGA3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Article

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-SYP1-miniSOG-T2A-mCherry

Plasmid

#50972

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)

Use

AAV

Tags

T2A-mCherry and miniSOG

Expression

Mutation

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLmMBP-11

Plasmid

#72349

Purpose

Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Use

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

Expression

Mammalian

Mutation

Promoter

CMV enhancer + chicken beta-actin promoter

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIV-EF1a-2A-ZsGreen

Plasmid

#231985

Purpose

Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

2A-ZsGreen

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Use

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-BirA*-pDEST-N-pcDNA5/FRT/TO

Plasmid

#118375

Purpose

Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-BirA*

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-FUW-PBRM1-pgk-puro

Plasmid

#85746

Purpose

tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-PLAA

Plasmid

#85669

Purpose

Expression of PLAA with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

PLAA (PLAA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP - FLAG-APEX2- hs Lamin B1

Plasmid

#139442

Purpose

APEX2-Lamin B1 expression plasmid

Depositor

Yixian Zheng

Article

Tran et al bioRxiv 2020.02.05.935635

Insert

LMNB1 (LMNB1 Human)

Use

Tags

3xGGGGS linker (between APEX2 and Lamin B1), APEX…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pY001 (pFnCpf1_full)

Plasmid

#69973

Purpose

Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

Plasmid

#176541

Purpose

human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB omegaRNA scaffold

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_OT1.0

Plasmid

#185384

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge