We narrowed to 13,726 results for: cas9

1. pLVX_DSB_Spectrum-V2

   Plasmid

   #192475

   Purpose

   Lentiviral DSB Spectrum V2 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro U6 sgRNA BfuAI stuffer

   Plasmid

   #50920

   Purpose

   U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL90002

   Plasmid

   #68261

   Purpose

   Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter: U6-26 (Arabidopsis thaliana)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnCasPA-BEC

   Plasmid

   #113349

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCBC-DT2T3

   Plasmid

   #50591

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, U6-29t plus, U6-1p, T3
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRCC-K

   Plasmid

   #81191

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX_DSB_Spectrum-V1

   Plasmid

   #192474

   Purpose

   Lentiviral DSB Spectrum V1 Reporter System

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   BFP1.2, incompleteGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458-mcherry

    Plasmid

    #161974

    Purpose

    Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Cas9-t2a-mcherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX_DSB_Spectrum-V3

    Plasmid

    #192476

    Purpose

    Lentiviral DSB Spectrum V3 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, mCherry, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCBC-MT1T2

    Plasmid

    #50593

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T1, gRNA scaffold, OsU3t plus, TaU3p, T2
    

    Use

    CRISPR; Pcr template

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VRQR-ABEmax

    Plasmid

    #119811

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTC223

    Plasmid

    #70019

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMpGE014

    Plasmid

    #108682

    Purpose

    All-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBK-YEE-BE3

    Plasmid

    #85177

    Purpose

    Expresses YEE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBHM2329

    Plasmid

    #171686

    Purpose

    pRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCR

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    C. neoformans U6

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGE013

    Plasmid

    #108681

    Purpose

    All-in-one genome editing vector in M. polymorpha (hygromycin resistant)

    Depositor

    Takayuki Kohchi, Shigeo Sugano

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge