We narrowed to 44,705 results for: cha
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Plasmid#102851PurposeA single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorInsertsp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B S. pyogenes, Synthetic, Human)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoterAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
his3MX6-ins3
Plasmid#195040PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertHis5+
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…Available SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-hGRIN2B-CAG-ps-SaCas9
Plasmid#102853PurposeA single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorInsertsa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Synthetic, Human)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoter;Available SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)
Plasmid#153441PurposeE. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc. XDocB binding mode B knock-out mutant
Tags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationArginine 140 and methionine 144 were mutated to a…PromoterT7 promoterAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT
Plasmid#111398PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT
Plasmid#111399PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346DPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT
Plasmid#111400PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT
Plasmid#111451PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540DAvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-FnCas9
Plasmid#130969Purpose3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNADepositorInsertFnCas9
UseCRISPRTags3xHA-NLS and NLS-T2A-EGFPExpressionMammalianPromoterCbhAvailable SinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en31FnCas9ABEmax8.17d
Plasmid#201956PurposeMammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNADepositorInsertbpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianPromoterCbhAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only