We narrowed to 30,616 results for: ica

1. pcDNA3.1/hChR2(H134R)-mCherry

   Plasmid

   #20938

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. human MMP-9 promoter

   Plasmid

   #53434

   Purpose

   promoter activity assay

   Depositor

   Douglas Boyd, Etty Benveniste

   Article

   Ma et al J Immunol. 2001 Nov 1;167(9):5150-9.

   Insert

   MMP-9 promoter (MMP9 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   MMP9

   Available since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB700 lenti gRNA Puro

   Plasmid

   #167911

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmyc-TNRC6B

   Plasmid

   #10978

   Depositor

   Thomas Tuschl

   Article

   Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

   Insert

   TNRC6B (TNRC6B Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTetR-LasR-pLuxR-GFP

   Plasmid

   #171159

   Purpose

   Sensing Device with Reporter protein

   Depositor

   Matthew Chang

   Article

   Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

   Inserts

   lasR
   gfp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLux and pTet

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU3-gRNA

   Plasmid

   #53063

   Purpose

   Transcription of guide RNA (gRNA) in rice cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   OsU3 promoter + gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   rice U3

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. wt Opa1

   Plasmid

   #204427

   Purpose

   Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

   Depositor

   Keir Neuman

   Article

   Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

   Insert

   hOpa1 isoform 1 (OPA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-Trx-mSA2

   Plasmid

   #52320

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, S tag, TEV site, and Thioredoxin

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Red Glifon 3000

   Plasmid

   #163116

   Purpose

   Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

   Depositor

   Takashi Tsuboi

   Article

   Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

   Insert

   Red Glifon 3000
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMH1105

    Plasmid

    #131864

    Purpose

    Produces holo-PhyB-AviTag in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

    Inserts

    PhyB(1-651)-AviTag-His6
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. nls-transposase-nls-pA in pT3TS-Dest

    Plasmid

    #140873

    Purpose

    Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nls-transposase-nls (Tol2)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-Puro Xlone-eGFP

    Plasmid

    #136936

    Purpose

    donor plasmid for inducible transgene expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    all-in-one tet-on system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAH-POLY

    Plasmid

    #64915

    Purpose

    Encodes a polyspecific aminoacyl-tRNA synthetase and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells

    Depositor

    Huiwang Ai

    Article

    Chen et al J Am Chem Soc. 2013 Oct 9;135(40):14940-

    Inserts

    Polyspecific aminoacyl-tRNA Synthetase
    Amber Suppression tRNA
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

    Plasmid

    #235150

    Purpose

    Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    HaloTag linked to SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Flag TSC2

    Plasmid

    #14129

    Depositor

    Brendan Manning

    Article

    Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

    Insert

    TSC2 (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBMN YFP-DHFR(DD)

    Plasmid

    #29326

    Depositor

    Thomas Wandless

    Article

    Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

    Insert

    YFP-DHFR(DD)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

    Promoter

    Available since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGADT7-αC

    Plasmid

    #197650

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-2 αC
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Ser677T substitution

    Promoter

    ADH1

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENT-L1-Kozak-lacZ-stop-L2

    Plasmid

    #186359

    Purpose

    Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli, Synthetic)
    

    Use

    Expression of lacz reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Kras-Src FRET biosensor

    Plasmid

    #78302

    Purpose

    FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

    Insert

    Src biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSGEMKv10.1

    Plasmid

    #85704

    Purpose

    Xenopus laevis oocyte expression of Kv10.1

    Depositor

    Luis Pardo

    Article

    Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

    Insert

    KCNH1 (KCNH1 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p212 pCMV-EGFP/RFP

    Plasmid

    #8389

    Purpose

    Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CROP_C9_Puro_TOP1

    Plasmid

    #183323

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pK18sB

    Plasmid

    #177838

    Purpose

    Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

    Type

    Empty backbone

    Use

    Bacterial gene replacement

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CROP_C9_Puro_AAVS1A

    Plasmid

    #183337

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    AAVS1A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-Tag2B-NCre

    Plasmid

    #106368

    Purpose

    N-terminal part of the split-Cre System

    Depositor

    Johannes Hirrlinger

    Article

    Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

    Insert

    NCre
    

    Use

    Cre/Lox

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CROP_C9_Puro_AAVS1B

    Plasmid

    #183338

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    AAVS1B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGDP1 vph

    Plasmid

    #112893

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    vph
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGDP3 vatD

    Plasmid

    #112885

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    vatD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGDP3 aph(9)-Ia

    Plasmid

    #112882

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    aph(9)-Ia
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKEK3395

    Plasmid

    #234079

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    YukonOFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET-22b(+) pCopA RBS30 mscarlet

    Plasmid

    #226370

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    mScarlet
    

    Use

    Tags

    6x Histag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA)

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

    Plasmid

    #198450

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CROP_C9_Puro_TOP2B

    Plasmid

    #183325

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CROP_C9_Puro_TOP2A

    Plasmid

    #183324

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    TOP2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGDP1 uvrA

    Plasmid

    #112892

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    uvrA-like protein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-hsRPA70 (MSW#473)

    Plasmid

    #208067

    Purpose

    Expression of GFP-tagged hsRPA1 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA1 (RPA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCGN-SRF

    Plasmid

    #11977

    Purpose

    For mammalian expression of human SRF with an HA tag

    Depositor

    Ron Prywes

    Article

    Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. dimericTomato-1xwGBD

    Plasmid

    #176099

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WAS (WAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET-AspS

    Plasmid

    #159618

    Purpose

    Expression plasmid for AcpS phosphopantetheinyl transferase

    Depositor

    Jun Yin, Christopher T Walsh

    Article

    Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

    Insert

    AcpS
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EGFP-Ki67-mCherry

    Plasmid

    #183748

    Purpose

    Expresses EGFP-Ki67-mCherry

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge