We narrowed to 11,691 results for: NSI

1. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

   Plasmid

   #191368

   Purpose

   Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dHgm4Cas13b-3xGFP-NLS
   

   Tags

   3xGFP

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

   Plasmid

   #191141

   Purpose

   Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587-W:EGFP
   

   Expression

   Mammalian

   Mutation

   UAG>UGG (stop>Trp)

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXWJ109As(RS-te-AAV)

   Plasmid

   #123156

   Purpose

   For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, ParsR, PhrpLE

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYFAC-CH3

   Plasmid

   #168979

   Purpose

   AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA

   Depositor

   Yit Heng Chooi

   Article

   Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

   Type

   Empty backbone

   Use

   Synthetic Biology; Heterologous expression in asp…

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRKVSV Rac

   Plasmid

   #50535

   Depositor

   Kenneth Yamada

   Article

   Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

   Insert

   small GTPase (RAC1 Human)
   

   Tags

   2X VSV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pYSD300

   Plasmid

   #212930

   Purpose

   Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   AGA1 (AGA1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHDAdVenus-geo-TK

   Plasmid

   #26423

   Depositor

   Kohnosuke Mitani

   Article

   Suzuki et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   PGK-b-geo, HSV-TK, CAG-Venus
   

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2-TTF-1-gRNA

   Plasmid

   #101857

   Purpose

   Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells

   Depositor

   David Mu

   Article

   Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

   Insert

   gRNA targeting TTF-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PXPR007 sgGFP-1

   Plasmid

   #74960

   Purpose

   Cas9 + sgGFP-1 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   GFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGG-cTP-TurboID-GFP

    Plasmid

    #209403

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Tags

    GFP

    Expression

    Plant

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available Since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB169

    Plasmid

    #37390

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    dynein light chain TcTex-type 3 (DYNLT3 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHygromycin (GB0211)

    Plasmid

    #68204

    Purpose

    Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hygromycin resistance gene coding region
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-FRB:Akt2

    Plasmid

    #72899

    Purpose

    Recruitable Akt2 (LocaTOR2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Serine/threonine protein kinase Bβ (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid

    #118407

    Purpose

    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-NES
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAB088h

    Plasmid

    #79215

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-TnCAD-F3
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLuciferase (GB0096)

    Plasmid

    #68201

    Purpose

    Provides the CDS of the firefly luciferase protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Luciferase coding region
    

    Use

    Luciferase and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SuExp-mPLD3

    Plasmid

    #201245

    Purpose

    express full length mouse PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (Pld3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDE1084

    Plasmid

    #168500

    Purpose

    expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

    Depositor

    Jean-Marc Daran

    Article

    Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

    Inserts

    Fncas12a
    T7RNA polymerase
    

    Use

    CRISPR

    Promoter

    TDH3 and TEF1

    Available Since

    Dec. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Myo 1d

    Plasmid

    #134906

    Purpose

    Expression of rat Myo 1d (Myr 4) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

    Insert

    Myo 1d (rat) with 3' UTR (Myo1d Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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