We narrowed to 11,283 results for: VARS

1. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

   Plasmid

   #192684

   Purpose

   Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. GB1-A11(2-196, G175R)-Strep

   Plasmid

   #196211

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G175R

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-A11(2-196, G189E)-Strep

   Plasmid

   #196212

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   G189E

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Luciferase

   Plasmid

   #213979

   Purpose

   Expresses Doxy Inducible Luciferase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   6xHis affinity tag

   Expression

   Bacterial and Mammalian

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

   Plasmid

   #101064

   Purpose

   AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2_L398T
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-v2 RsTAL At4CL

   Plasmid

   #126524

   Purpose

   expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

   Depositor

   Andrew Woolley

   Article

   Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

   Inserts

   tyrosine ammonia lyase
   p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.SF-iGluSnFR.A184S

   Plasmid

   #106198

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Mutation

   GltI: A184S

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-ABE7

    Plasmid

    #193644

    Purpose

    Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA-TadA*7.10, EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.CAG-FLEX.SF-iGluSnFR.A184S

    Plasmid

    #106186

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    CAG

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS 3xFLAG Mbnl2

    Plasmid

    #217032

    Purpose

    Overexpression of Mbnl2 in mammalian cells

    Depositor

    Chaolin Zhang

    Article

    Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

    Insert

    Mbnl2 (Mbnl2 Mouse)
    

    Expression

    Mammalian

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP

    Plasmid

    #216475

    Purpose

    lentiviral dual expression of YAP5SA and eGFP

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    YAP5SA
    eGFP
    

    Use

    Lentiviral

    Mutation

    S61A, S109A, S127A, S164A, S381A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist-SARS-CoV Δ18

    Plasmid

    #169465

    Purpose

    Encodes SARS-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV Spike truncated to remove 18 amino acids (S )
    

    Expression

    Mammalian

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-LTK-V5/HIS

    Plasmid

    #200116

    Purpose

    Expression of human LTK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human LTK receptor tyrosine kinase (LTK Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT_KCNQ3

    Plasmid

    #173163

    Purpose

    To generate Flp-In CHO-KCNQ3 stable cells

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2022 Mar 8;7(5):e156314. do

    Insert

    KCNQ3 (KCNQ3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNMSB84

    Plasmid

    #199309

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
    

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

    Promoter

    flp-18

    Available Since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1-RnKIF5C(1-560)-Halo-Flag

    Plasmid

    #188071

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAT100

    Plasmid

    #180512

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_HCK-1/1

    Plasmid

    #91273

    Purpose

    Protein expression and purification of human SH3 domain construct HCK-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCK-1/1 (HCK Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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