We narrowed to 13,996 results for: eng

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pCarCBA

Plasmid

#117754

Purpose

Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA)

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago2_3

Plasmid

#73531

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago2_4

Plasmid

#73532

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only
pJKW 0643

Plasmid

#74121

Purpose

Photinus pyralis luciferin sulfotransferase LST - pHis8-4

Depositor

Jing-Ke Weng

Article

Fallon et al Biochemistry. 2016 May 26.

Insert

Photinus pyralis firefly luciferin sulfotransferase

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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TAL3105

Plasmid

#41279

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-irf6-Right (irf6 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3104

Plasmid

#41278

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-irf6-Left (irf6 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
drd4a_R (TAL 3007)

Plasmid

#35999

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting drd4a (drd4a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pDUET-ctCPR-trAMO

Plasmid

#20112

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and truncated amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pX330-CANX

Plasmid

#227279

Purpose

Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CANX (CANX Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-[EF1α-CD19 4-1BB]

Plasmid

#218336

Purpose

Lentiviral transfer plasmids for mammalian expression CD19 CAR

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-gst-xr

Plasmid

#214761

Purpose

Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

Depositor

Pimchai Chaiyen

Article

Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

Insert

glutathione S-transferases fused with xylose reductase

Tags

Glutathione S-transferases

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_BGL1

Plasmid

#183735

Purpose

Expresses Aspergillus aculeatus β-glucosidase (BGL1)

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

β-glucosidase (AaBGL1)

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.155_LV-Puro-T2A-mTagBFP2

Plasmid

#205553

Purpose

Express puromycin resistance marker and mTagBFP2

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Puro-T2A-mTagBFP2

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-PA-mCherry

Plasmid

#138251

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHBS1410 GFP-TDP43 F-S

Plasmid

#118799

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All F to S in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3250

Plasmid

#42713

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-esr1-left (esr1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3366

Plasmid

#42829

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox1a-left (sox1a Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
TAL3251

Plasmid

#42714

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-esr1-right (esr1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only