We narrowed to 19,819 results for: Cre
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Plasmid#139858PurposeLentiviral expression of human DDRGK1-K224R-HADepositorAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only
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pX330-Flag-WT_SpCas9 (without sgRNA; with silent mutations)
Plasmid#126753PurposeExpression plasmid for human codon-optimized WT SpCas9 (without U6-sgRNA coding sequence, with silent mutations)DepositorInsertWT SpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-jph1a
Plasmid#109497PurposeMuscle specific zebrafish expression of mKate2 fused to junctophilin 1aDepositorInsertjph1a (jph1a Zebrafish)
UseZebrafish plasmidsTagsmKate2MutationPlease see Depositor CommentsAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ARF1-DN
Plasmid#109590PurposeMultisite gateway vector for 3' tagging with dominant negative ARF1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-ARF6-DN
Plasmid#109592PurposeMultisite gateway vector for 3' tagging with dominant negative ARF6DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-SERCA1
Plasmid#109552PurposeMultisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1DepositorInsertsarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ARF1
Plasmid#109535PurposeMuscle specific zebrafish expression of mKate2 fused to ARF1DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-Cav3-mKate2
Plasmid#109518PurposeMuscle specific zebrafish expression of mKate2 fused to Caveolin3DepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Dnm2a-DN
Plasmid#109520PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Dynamin2aDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GS2.1/EC
Plasmid#132568PurposesgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).DepositorInsertegg cell promoter, Cas9-mApple, pds3 sgRNA
UseCRISPR and Synthetic BiologyExpressionPlantPromoterA. thaliana egg cell promoterAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph20-Stg
Plasmid#133035PurposeExpresses Xph20-Stg in bacteriaDepositorInsertXph20-Stg
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-ETWV
Plasmid#133036PurposeExpresses Xph18-ETWV in bacteriaDepositorInsertXph18-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph18-Stg
Plasmid#133037PurposeExpresses Xph18-Stg in bacteriaDepositorInsertXph18-Stg
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph15-ETWV
Plasmid#133038PurposeExpresses Xph15-ETWV in bacteriaDepositorInsertXph15-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialMutationS63KPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pbIG-Xph0-ETWV
Plasmid#133039PurposeExpresses Xph0-ETWV in bacteriaDepositorInsertXph0-ETWV
TagsAviTag, His10, and TEV protease cleavage siteExpressionBacterialPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-TAT-Xph20-ETWV
Plasmid#133040PurposeExpresses TAT-Xph20-ETWV in bacteriaDepositorInsertXph20-ETWV
TagsHis8, TAT, and TEV protease cleavage siteExpressionBacterialPromoterT7Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationMutationThree Mets (133, 138, 186) to IleuPromoterT7Available SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
563aa ELL2 pEF4B
Plasmid#127273PurposeExpresses 563aa human WT ELL2DepositorInsertELL2 truncated at SphI (ELL2 Human)
ExpressionMammalianMutationContains 564 N-terminal amino acids ELL2, termina…PromoterpEF1aAvailable SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2
Plasmid#121123Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR (cdk2 Zebrafish)
ExpressionBacterialAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W
Plasmid#105041PurposeLentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W
Plasmid#105042PurposeLentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tcf7l1-g1)-PGKpuroBFP-W
Plasmid#105015PurposeLentiviral gRNA plasmid targeting mouse Tcf7l1 , co-expression of TagBFPDepositorInsertgRNA targeting Tcf7l1 (Tcf7l1 Mouse)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W
Plasmid#105018PurposeLentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W
Plasmid#105020PurposeLentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-SENP8 sg2R-P2A-Hygro
Plasmid#124300PurposeLentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertSENP8 (SENP8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2
Plasmid#127270PurposeFor in vitro translation of human WT ELL2 with internal HA tagDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationWT Mets and HA tag at SphI site (8th Met)PromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged mid ELL2
Plasmid#127274PurposeExpresses human ELL2 with HA tag in the middle that does not disrupt functionDepositorInsertELL2 with HA at XbaI (ELL2 Human)
ExpressionMammalianMutationHA inserted at XbaI after Met 186PromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu ELL2
Plasmid#127260PurposeFor in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met138 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet138 to Ileu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met186ILeu ELL2
Plasmid#127261PurposeFor in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met186 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet186 to Ileu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met138ILeu, Met186ILeu ELL2
Plasmid#127265PurposeFor in vitro translation of human ELL2 with Met138ILeu, Met186ILeuDepositorInsertELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (138,186) to ILeu QuikchangePromoterT7Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu ELL2
Plasmid#127259PurposeFor in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met133 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet133 to Ileu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met133ILeu, Met186ILeu ELL2
Plasmid#127264PurposeFor in vitro translation of human ELL2 with Met133ILeu, Met186ILeuDepositorInsertELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
UseIn vitro translationMutationTwo Mets (133,186 )to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met331Leu ELL2
Plasmid#127262PurposeFor in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met331 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet331 to ILeu QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
COOHend ELL2
Plasmid#127255PurposeExpresses the C terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (C-terminal end) (ELL2 Human)
UseOtherExpressionMammalianMutationcontains only the last 389 aaPromoterpEF1aAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Met1ILeu ELL2
Plasmid#127258PurposeFor in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiationDepositorInsertELL2 (Met1 to Ileu) (ELL2 Human)
UseIn vitro translationMutationMet1 to Ileu by QuikchangePromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
ELL2mRNA+polyA
Plasmid#127256PurposeFor in vitro translation of human ELL2 with 30 nt pA tailDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationshortens 3'UTR to 40 bp adds A30 tailPromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
NH2end ELL2
Plasmid#127252PurposeExpresses the N terminal segment of human ELL2 in mammalian cellsDepositorInsertELL2 (1-287) (ELL2 Human)
UseOtherTagsHis6XExpressionMammalianMutationcontains only amino acids 1-289PromoterpEF1aAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2
Plasmid#127268PurposeFor in vitro translation of WT ELL2 with HA tag inserted near N-termDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationELL2mRNA+polyA with HA tag inserted at aa12 betwe…PromoterT7Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR
Plasmid#121836PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB
Plasmid#121837PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only