We narrowed to 9,521 results for: try

1. pRK5-FLAG-Sestrin2-S190W

   Plasmid

   #73670

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

   Insert

   Sesn2 (SESN2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S190W changed serine 190 to tryptophan

   Promoter

   CMV

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pT7_circRAJ31_C873U

   Plasmid

   #69932

   Purpose

   Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.

   Depositor

   Alfonso Jaramillo

   Article

   Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

   Insert

   pT7_circRAJ31_C873U
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Wnt7B STOP

   Plasmid

   #35878

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt7B (Wnt7b Human)
   

   Use

   Entry vector

   Mutation

   None

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. DHRS4

   Plasmid

   #39071

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DHRS4 (DHRS4 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. DT036A

   Plasmid

   #159396

   Purpose

   Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter

   Depositor

   Sheref Mansy

   Article

   Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

   Insert

   pr.tet-sfGFP
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   tet promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGEV_4'_+nls-mCherry-Flag-_+1_BK+

   Plasmid

   #49313

   Purpose

   Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-mCherry-Flag
   

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. C511-E02: attB5r-NanoLuc-nostop-attB1r

   Plasmid

   #162938

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   Nanoluciferase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. C413-E33: attB4-UbCp>-attB1r

   Plasmid

   #162925

   Purpose

   Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   human Ubiquitin C promoter
   

   Use

   Synthetic Biology

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. C232-E29: attB2r-mClover3-attB3

   Plasmid

   #162906

   Purpose

   Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   monomeric Clover3 fluorescent protein
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. C232-E30: attB2r-mRuby3-attB3

    Plasmid

    #162907

    Purpose

    Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    monomeric Ruby3 fluorescent protein
    

    Use

    Synthetic Biology

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Wnt9A STOP

    Plasmid

    #35881

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt9A (WNT9A Human)
    

    Use

    Entry vector

    Mutation

    Silent Mutation A (543) --> G

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. C234-E25: attB2r-SNAP-attB3

    Plasmid

    #162914

    Purpose

    Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    SNAP tag
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYZ221

    Plasmid

    #132952

    Purpose

    crRNA entry vector of Prrk1-Not1-HHR with FnCas12a in Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    FnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    adh1

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYZ675

    Plasmid

    #132954

    Purpose

    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    nucleoplasmin NLS

    Promoter

    adh1

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYZ694

    Plasmid

    #132956

    Purpose

    crRNA entry vector of Prrk1-Not1-HHR with dFnCas12a-Clr4 in Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS and nucleoplasmin NLS

    Promoter

    adh1

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFTK047

    Plasmid

    #171319

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    DsRed-NLS fluorescent reporter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFTK033

    Plasmid

    #171305

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    CPnirA AN0098 core promoter (for fusion)
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFTK026

    Plasmid

    #171298

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    CPnirA AN0098 (no UAS) core promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFTK013

    Plasmid

    #171285

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    PpcbC Pc21g21380 promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFTK011

    Plasmid

    #171283

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    PpcbAB Pc21g21390 promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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