Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,181 results for: nes

Clear keywords
Showing: 5521 - 5540 of 16181 results

  10. pAT15547_PEmax-SuperFi-Cas9

    Plasmid
    #184377
    Purpose
    Mammalian expression of SuperFi-Cas9 PEmax prime editor
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-SuperFi-Cas9
    Use
    CRISPR
    Tags
    SV40 NLS and c-myc NLS
    Expression
    Mammalian
    Mutation
    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184377-plasmid-map-sequence-id-362139

    Enlarge

  11. hAID-BE3 (pRZ352)

    Plasmid
    #131316
    Purpose
    CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131316-plasmid-map-sequence-id-254676

    Enlarge

  12. pTBL680 CHYRON3 integration construct

    Plasmid
    #126448
    Purpose
    To integrate the CHYRON3 locus at AAVS1 in human cells.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    pU6/3xLacO-CHYRON3 hgRNA
    pCMV-puro
    Expression
    Mammalian
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    CMV and human U6/3xLacO
    Available since
    June 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    126448-plasmid-map-sequence-id-246061

    Enlarge

  13. pCOCK CM-R03

    Plasmid
    #46567
    Purpose
    Mammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Syst Synth Biol. 2011 Dec;5(3-4):131-8.
    Insert
    CMV mutant promoter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    Mutant version of CMV promoter (refer to Table 1 …
    Available since
    Sept. 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pX330-Flag-eSpCas9 (without sgRNA)

    Plasmid
    #92354
    Purpose
    Expression plasmid for human codon-optimized high-fidelity eSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG-NLS-Streptococcus pyogenes enhanced Cas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92354-plasmid-map-sequence-id-197504

    Enlarge

  15. Emerald-pBAD

    Plasmid
    #54776
    Purpose
    Localization: Protein Expression Vector , Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and Emerald
    Expression
    Bacterial
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. rsGFP1-pBAD

    Plasmid
    #54851
    Purpose
    Localization: Protein Expression Vector , Excitation: 493, Emission: 510. Also called rsEGFP1
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and rsGFP1
    Expression
    Bacterial
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. Clover2-pBAD

    Plasmid
    #54576
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    6xHis and Clover2
    Expression
    Bacterial
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. pAGT9569

    Plasmid
    #219431
    Purpose
    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS
    Depositor
    Tom Schreiber
    Publication
    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000
    Insert
    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    PapE-4LF2
    Expression
    Plant
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    219431-plasmid-map-sequence-id-436703

    Enlarge

  19. pSc1

    Plasmid
    #80436
    Purpose
    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell
    Depositor
    Ervin Welker
    Publication
    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sp Cas9 gRNA
    eGFP
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80436-plasmid-map-sequence-id-151445

    Enlarge

  20. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

    Enlarge

Showing: 5521 - 5540 of 16181 results