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pCEP4-DU422gp160

Plasmid

#100926

Purpose

Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422.1) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH111gp160

Plasmid

#123268

Purpose

Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1

Plasmid

#232746

Purpose

IVT template for making modRNA encoding Bxb1

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pRN119

Plasmid

#176884

Purpose

Encodes STM and WUS2 for ectopic meristem induction in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP, Nos::WUS2:pinII, and 35:STM:35S

Expression

Plant

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJAT11

Plasmid

#204290

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

ie1-DsRed

Use

CRISPR

Promoter

ie1

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-B41gp160

Plasmid

#123247

Purpose

Mammalian expression plasmid for Env from the B41 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (B41) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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SB_convex

Plasmid

#110104

Purpose

pBXPHM3 with scaffold Sybody of the convex library

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Insert

scaffold Sybody of the convex library

Tags

His

Expression

Bacterial

Promoter

PBAD

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-cyto-KB1691-Nluc

Plasmid

#204326

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

cytosolic KB1691-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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CL7-LbCas12a

Plasmid

#164658

Purpose

Bacterial expression of LbCas12a using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro Rb shRNA

Plasmid

#10670

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Rb shRNA (RB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA3

Plasmid

#153111

Purpose

Expression of Flag tagged human FOXA3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA3 (FOXA3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-smyhc1

Plasmid

#126748

Purpose

Zebrafish slow myosin heavy chain promoter. Clone 667

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

smyhc1 (smyhc1 Zebrafish)

Use

Multisite gateway entry clone

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRG705_lexO-AsiSI_LexA-TF_LEU2MX

Plasmid

#154817

Purpose

Inducible expression of AsiSI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AsiSI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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human p53-(311-393)

Plasmid

#24869

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 311-393.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
CMV-mito-Citroff1-S245

Plasmid

#134306

Purpose

mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab2b

Plasmid

#203692

Purpose

Expresses N8C_Fab2b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab2b

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLz37_CB_operon_CsoS2_N1-N4

Plasmid

#140856

Purpose

Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Expression

Bacterial

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGMC00029 (aka pRC0610)

Plasmid

#200834

Purpose

Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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