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pLenti-CaMaKIIalpha-mKO2

Plasmid

#86156

Purpose

Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.

Depositor

James Ellis

Article

Rodrigues et al Cell Rep. 2016 Oct 11;17(3):720-734. doi

Insert

mKO2

Use

Lentiviral

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Ub-UBB+1

Plasmid

#23967

Depositor

Nico Dantuma

Article

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pCSC-ASCL1-IRES-Sox11

Plasmid

#182234

Purpose

To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

Depositor

Chun-Li Zhang

Article

Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

Insert

ASCL1-IRES-Sox11

Use

Lentiviral

Promoter

CMV

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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Non-targeting CRISPRi plasmid

Plasmid

#154891

Purpose

Human non-targeting CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

Insert

Nontargeting gRNA

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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AV4-EF1-GFP

Plasmid

#124971

Purpose

Control for AV4-EF1-NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

GFP

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBK1302-AAV-EFSNC-dCjCas9-KRAB

Plasmid

#223147

Purpose

Expression of KRAB with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1312-AAV-EFSNC-dSaCas9-KRAB

Plasmid

#223157

Purpose

Expression of KRAB with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHA841 - hrp-1p::hrp-1HsLCWT

Plasmid

#139198

Purpose

Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

last exon replaced with correspoding human sequen…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA842 - hrp-1p::hrp-1HsLCD290V

Plasmid

#139199

Purpose

Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

D290V, last exon replaced with correspoding human…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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