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Plasmid#110070PurposeExpress Halo-Tev-Flag-Ube2v2 (human) in mammalian cellsDepositorInsertHalo-Tev-Flag-Ube2v2 (UBE2V2 Human)
UseTagsHalo-TEV-FlagExpressionMammalianMutationPromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
DL017:CMV::HaloTag-iPAK4
Plasmid#177882PurposeHT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synapsin-WHaloCaMP1a-EGFP
Plasmid#205307PurposeProduction of AAV particles encoding WHaloCaMP1a-EGFP from the synapsin promoterDepositorInsertWHaloCaMP1a-EGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHaloTag141/145
Plasmid#194660PurposeProtein production of circular permuted HaloTag (cpHaloTag141/145) in bacteriaDepositorInsertcpHaloTag141/145
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHaloTag154/156
Plasmid#194661PurposeProtein production of circular permuted HaloTag (cpHaloTag154/156) in bacteriaDepositorInsertcpHaloTag154/156
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-A2A-adenosine receptor
Plasmid#66995PurposeA2A-adenosine receptor tagged with HaloTag on amino terminusDepositorInsertHaloTag-A2A-adenosine receptor (ADORA2A Human)
UseTagsHuman Growth hormone Signal Sequence- HaloTagExpressionMammalianMutationThe signal sequence from human growth hormone was…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA1-scFV-Halo
Plasmid#230065PurposeExpresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.DepositorInsertAnti-GluA1 single chain variable fragment (scFv)
UseTagsHaloTagExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209665PurposeExpression of iATPSnFR2, low affinityDepositorInsertiATPSnFR2.A95A.A119L.HaloTag
UseAAVTagsExpressionMutationA95A.A119LPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7
Plasmid#175536PurposeExpression of HaloTag7 at the at the Gogli apparatus of the mammalian cellsDepositorInsertBeta4Gal-HaloTag7
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_b4g-HaloTag9
Plasmid#175546PurposeExpression of HaloTag9 at the at the Gogli apparatus of the mammalian cellsDepositorInsertBeta4Gal-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-BRAF-REG-Halo
Plasmid#194771PurposeMammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.DepositorInsertBRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMV51Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusTagsExpressionMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV ORANGE Dlg4-HaloTag KI
Plasmid#139656PurposeEndogenous tagging of PSD95: C-terminal (amino acid position: R721)DepositorInsertgRNA and HaloTag donor
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
SFFV-HALO-CTERM-GFP
Plasmid#185767PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-HALO-NTERM-GFP
Plasmid#185762PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-H2AZ-halo
Plasmid#197907Purposesingle-particle tracking for H2AZDepositorInsertH2az2 (H2az2 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-mCherry-Halo-YAP1
Plasmid#128336PurposeRetrovirus construct to express mCherry-Halo-YAP1DepositorInsertYAP1 (YAP1 Human)
UseRetroviralTagsmCherry and Halo-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT006-GCN4-HaloPM
Plasmid#163386PurposeTo express 4X sGCN4 module in the G-baToN systemDepositorInsert4XGCN4-Halotag-PDGFRtm-2A-mCherry (GCN4-HaloPM)
UseLentiviralTagsmCherryExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-end
Plasmid#71512PurposeMammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.DepositorInsertConnexin 35
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).iATPSnFR2.S29W.A95K.HaloTag
Plasmid#209659PurposeExpression of iATPSnFR2, high affinityDepositorInsertiATPSnFR2.S29W.HaloTag
UseAAVTagsExpressionMutationS29W.A95KPromoterGFAPAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only