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We narrowed to 5,049 results for: golden gate

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Showing: 1351 - 1400 of 5049 results

  1. pKS1173

    Plasmid
    #239507
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239507-plasmid-map-sequence-id-478369

    Enlarge

  2. pKS1140

    Plasmid
    #239501
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239501-plasmid-map-sequence-id-475913

    Enlarge

  3. pKS1170

    Plasmid
    #239504
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239504-plasmid-map-sequence-id-475911

    Enlarge

  4. pKS1577

    Plasmid
    #239525
    Purpose
    Express Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239525-plasmid-map-sequence-id-475912

    Enlarge

  5. pKS1141

    Plasmid
    #239502
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239502-plasmid-map-sequence-id-475904

    Enlarge

  6. pKS1461

    Plasmid
    #239515
    Purpose
    Express the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    ScErg12p-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239515-plasmid-map-sequence-id-475897

    Enlarge

  7. pKS1171

    Plasmid
    #239505
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239505-plasmid-map-sequence-id-475889

    Enlarge

  8. pKS1172

    Plasmid
    #239506
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239506-plasmid-map-sequence-id-475884

    Enlarge

  9. pKS1520

    Plasmid
    #239520
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239520-plasmid-map-sequence-id-475881

    Enlarge

  10. pKS1138

    Plasmid
    #239499
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239499-plasmid-map-sequence-id-475874

    Enlarge

  11. pKS1428

    Plasmid
    #239513
    Purpose
    Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    AtUTR1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239513-plasmid-map-sequence-id-475875

    Enlarge

  12. pKS1139

    Plasmid
    #239500
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239500-plasmid-map-sequence-id-475873

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  13. pAIP4_2RH3

    Plasmid
    #234219
    Purpose
    Heterologous protein expression of protein virC2 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2RH3 (virC2 Agrobacterium tumefaciens)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234219-plasmid-map-sequence-id-464184

    Enlarge

  14. pAIP4_4REH

    Plasmid
    #234195
    Purpose
    Heterologous protein expression of major latex-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4REH
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234195-plasmid-map-sequence-id-464160

    Enlarge

  15. pAIP4_4ZHW

    Plasmid
    #234158
    Purpose
    Heterologous protein expression of YfiB in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ZHW (yfiB Pseudomonas aeruginosa PAO1)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234158-plasmid-map-sequence-id-464124

    Enlarge

  16. pAIP4_1XSZ

    Plasmid
    #234151
    Purpose
    Heterologous protein expression of RalF in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1XSZ (ralF Legionella pneumophila)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234151-plasmid-map-sequence-id-464117

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  17. pAIP4_2D5J

    Plasmid
    #234143
    Purpose
    Heterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2D5J
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234143-plasmid-map-sequence-id-464109

    Enlarge

  18. L1B39 - pMUTE-Bxb1-tUBQ10

    Plasmid
    #219704
    Purpose
    Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pMUTE:Bxb1:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219704-plasmid-map-sequence-id-435734

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  19. PROM_10

    Plasmid
    #201301
    Purpose
    D10: Promoter of TraesCS3D02G517600 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D10: Promoter of TraesCS3D02G517600
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201301-plasmid-map-sequence-id-410981

    Enlarge

  20. pLM001

    Plasmid
    #201311
    Purpose
    AvrSr50 (with stop codon) as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr50
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201311-plasmid-map-sequence-id-410106

    Enlarge

  21. pLM002

    Plasmid
    #201312
    Purpose
    AvrSr50 (no stop codon) as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr50
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201312-plasmid-map-sequence-id-410095

    Enlarge

  22. PROM_3

    Plasmid
    #201296
    Purpose
    D3: Promoter of TraesCS3A02G510700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D3: Promoter of TraesCS3A02G510700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201296-plasmid-map-sequence-id-409521

    Enlarge

  23. PROM_12

    Plasmid
    #201303
    Purpose
    D12: Promoter of TraesCS4D02G131100 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D12: Promoter of TraesCS4D02G131100
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201303-plasmid-map-sequence-id-409517

    Enlarge

  24. PROM_4

    Plasmid
    #201297
    Purpose
    D4: Promoter of TraesCS3B02G552700 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D4: Promoter of TraesCS3B02G552700
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201297-plasmid-map-sequence-id-409516

    Enlarge

  25. PROM_6

    Plasmid
    #201299
    Purpose
    D6: Promoter of TraesCS3B02G578800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D6: Promoter of TraesCS3B02G578800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201299-plasmid-map-sequence-id-409515

    Enlarge

  26. PROM_5

    Plasmid
    #201298
    Purpose
    D5: Promoter of TraesCS3B02G553900 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D5: Promoter of TraesCS3B02G553900
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201298-plasmid-map-sequence-id-409513

    Enlarge

  27. PROM_1

    Plasmid
    #201294
    Purpose
    D1: Promoter of TraesCS1A02G332800 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D1: Promoter of TraesCS1A02G332800
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201294-plasmid-map-sequence-id-409512

    Enlarge

  28. PROM_7

    Plasmid
    #201300
    Purpose
    D7: Promoter of TraesCS3D02G316100 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D7: Promoter of TraesCS3D02G316100
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201300-plasmid-map-sequence-id-409511

    Enlarge

  29. PROM_2

    Plasmid
    #201295
    Purpose
    D2: Promoter of TraesCS2B02G533000 as Golden Gate compatible MoClo PROM part
    Depositor
    Benjamin Schwessinger
    Insert
    D2: Promoter of TraesCS2B02G533000
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201295-plasmid-map-sequence-id-409509

    Enlarge

  30. pPD698 ZF172i

    Plasmid
    #138791
    Purpose
    Constitutive expression of ZF172i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF172i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138791-plasmid-map-sequence-id-320374

    Enlarge

  31. pPD699 ZF173i

    Plasmid
    #138792
    Purpose
    Constitutive expression of ZF173i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF173i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138792-plasmid-map-sequence-id-320375

    Enlarge

  32. pPD686 ZF13i

    Plasmid
    #138774
    Purpose
    Constitutive expression of ZF13i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF13i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138774-plasmid-map-sequence-id-320235

    Enlarge

  33. pPD687 ZF14i

    Plasmid
    #138775
    Purpose
    Constitutive expression of ZF14i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF14i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138775-plasmid-map-sequence-id-320244

    Enlarge

  34. pPD688 ZF21i

    Plasmid
    #138776
    Purpose
    Constitutive expression of ZF21i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF21i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138776-plasmid-map-sequence-id-320248

    Enlarge

  35. pPD689 ZF36i

    Plasmid
    #138777
    Purpose
    Constitutive expression of ZF36i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF36i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138777-plasmid-map-sequence-id-320252

    Enlarge

  36. pPD690 ZF42i

    Plasmid
    #138779
    Purpose
    Constitutive expression of ZF42i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF42i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138779-plasmid-map-sequence-id-320259

    Enlarge

  37. pPD691 ZF54i

    Plasmid
    #138781
    Purpose
    Constitutive expression of ZF54i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF54i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138781-plasmid-map-sequence-id-320262

    Enlarge

  38. pPD692 ZF55i

    Plasmid
    #138782
    Purpose
    Constitutive expression of ZF55i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF55i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138782-plasmid-map-sequence-id-320269

    Enlarge

  39. pPD693 ZF62i

    Plasmid
    #138783
    Purpose
    Constitutive expression of ZF62i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF62i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138783-plasmid-map-sequence-id-320273

    Enlarge

  40. pPD252 ZF92i

    Plasmid
    #138784
    Purpose
    Constitutive expression of ZF92i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF92i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138784-plasmid-map-sequence-id-320274

    Enlarge

  41. pPD694 ZF93i

    Plasmid
    #138785
    Purpose
    Constitutive expression of ZF93i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF93i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138785-plasmid-map-sequence-id-320275

    Enlarge

  42. pPD253 ZF97i

    Plasmid
    #138786
    Purpose
    Constitutive expression of ZF97i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF97i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138786-plasmid-map-sequence-id-320353

    Enlarge

  43. pPD695 ZF129i

    Plasmid
    #138787
    Purpose
    Constitutive expression of ZF129i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF129i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138787-plasmid-map-sequence-id-320354

    Enlarge

  44. pPD696 ZF150i

    Plasmid
    #138788
    Purpose
    Constitutive expression of ZF150i under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    ZF150i
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138788-plasmid-map-sequence-id-320369

    Enlarge

  45. U9m

    Plasmid
    #121022
    Purpose
    MoClo golden gate assembly BC part for BCD22 (very low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD22
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121022-plasmid-map-sequence-id-236185

    Enlarge

  46. T1m

    Plasmid
    #121028
    Purpose
    MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).
    Depositor
    Richard Murray
    Insert
    T500_noGap, short attachment (T11)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121028-plasmid-map-sequence-id-245901

    Enlarge

  47. C113m

    Plasmid
    #121012
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Cas9
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121012-plasmid-map-sequence-id-236320

    Enlarge

  48. U2m

    Plasmid
    #121015
    Purpose
    MoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD11
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121015-plasmid-map-sequence-id-236174

    Enlarge

  49. U3m

    Plasmid
    #121016
    Purpose
    MoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD13
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121016-plasmid-map-sequence-id-236176

    Enlarge

  50. U5m

    Plasmid
    #121018
    Purpose
    MoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    BCD14
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121018-plasmid-map-sequence-id-238061

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