Addgene

We narrowed to 823 results for: 3xha

Showing: 541 - 560 of 823 results

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pGG-TurboID-GFP

Plasmid

#209399

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Use

Tags

GFP

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0019

Plasmid

#205992

Purpose

pNpuDnaE(C)_HygR(53-341)_3HA

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-HygR

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pT22i5uasH3En2SE4P3Che(#1212)

Plasmid

#184098

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Insert

3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Expression

Mutation

chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…

Promoter

5xUAS

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-miniTurbo-NLS-Blast

Plasmid

#232347

Purpose

Lentiviral control vector expressing miniTurboNLS without dCas9

Depositor

Ali Shilatifard

Article

Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

Insert

miniTurbo

Use

Lentiviral

Tags

3xHA and 3xSV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pC0046-EF1a-PspCas13b-NES-HIV

Plasmid

#103862

Purpose

Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-VP64

Plasmid

#50918

Purpose

Constitutive dCas9-VP64 lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGG-TurboID-3XNLS

Plasmid

#209392

Purpose

Transiently expressing TurboID fused with 3X nuclear localization signal (NLS) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID-3XNLS

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-gate-pGK-HYG

Plasmid

#107396

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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p1195-pssAAV.U1a.hNme2Cas9

Plasmid

#129534

Purpose

AAV vector expressing Nme2Cas9

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

human codon-optimized Nme2Cas9

Use

AAV

Tags

2xNLS and NLS-3xHA-NLS

Expression

Mutation

Promoter

U1a

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-myc T58A

Plasmid

#19763

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+_HA-mAID-nanobody (Kless)

Plasmid

#117718

Purpose

mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

3xHA

Expression

Mammalian

Mutation

codon-optimized and lysine-less mAID for murine e…

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Use

Tags

3xHA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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TAp63gamma-(3X)HA

Plasmid

#26977

Depositor

David Sidransky

Article

Upadhyay et al Biochem Biophys Res Commun. 2007 May 18.

Insert

TAp63gamma (TP63 Human)

Use

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-3HA

Plasmid

#39351

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA and kanMX6 module - reverse orientation

Expression

Mutation

Promoter

Available since

Jan. 10, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGG-GFP-PM-TurboID

Plasmid

#209402

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

cTP-3XHA-TurboID

Use

Tags

GFP

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-PM-TurboID

Plasmid

#209395

Purpose

Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

PM-3XHA-TurbOID

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-AkCas12b-2AeGFP

Plasmid

#121946

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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