We narrowed to 8,828 results for: Pol

1. pZH20 Dual G-less containing yCYC1 polyA

   Plasmid

   #231646

   Purpose

   yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

   Depositor

   Jesper Svejstrup

   Article

   Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

   Insert

   CYC1 polyA termination region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

   Plasmid

   #215699

   Purpose

   Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

   Plasmid

   #208966

   Purpose

   Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

   Promoter

   CMV and T7

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

   Plasmid

   #208974

   Purpose

   Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

   Plasmid

   #208973

   Purpose

   Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]

   Plasmid

   #198381

   Purpose

   Expression of SYPB::CRY2olig(535) in neurons of Danio rerio

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   UAS:sypb-egfp-cry2
   

   Use

   Tol2

   Tags

   EGFP

   Expression

   Mutation

   D387A

   Promoter

   UAS

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-EGFP barcode-9-SV40 polyA

   Plasmid

   #190873

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

   Plasmid

   #190903

   Purpose

   AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   GFP, sgHTT and sgCas9 (HTT Human)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EFS and U6

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIneo-TISU-RLuc-6xbsMS2-polyA-Malat_AC

    Plasmid

    #148436

    Purpose

    Mammalian Expression of 6xbsMS2-polyA-Malat

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    6xbsMS2-polyA-Malat
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1C-no5UTR-Fluc-5BoxB-PolyA-HhR_AD

    Plasmid

    #148499

    Purpose

    Insect Expression of 5BoxB-PolyA-HhR

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    5BoxB-PolyA-HhR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-EGFP barcode-7-SV40 polyA

    Plasmid

    #190871

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-CAG-EGFP barcode-8-SV40 polyA

    Plasmid

    #190872

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode8
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-EGFP barcode-6-SV40 polyA

    Plasmid

    #190870

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode6
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJET-63-SFFV-T2A-BlaR-polyA-R11a

    Plasmid

    #179894

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJET-61-SFFV-T2A-PuroR-polyA-R11a

    Plasmid

    #179892

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid

    #179893

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid

    #179895

    Purpose

    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-IRES-GFP-polar-bear-Caspase-1/4b

    Plasmid

    #183370

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4b
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hygro-Ef1A-Myc-POLB(PAMmut)

    Plasmid

    #176150

    Purpose

    N-terminus Myc-tagged POLB containing a mutation in the PAM site used by POLB gRNA1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by POLB gRNA1

    Promoter

    EF1A

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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