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pGEX-mdm2/2702

Plasmid

#11492

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
p23-DDX21 DEV

Plasmid

#128805

Purpose

overexpression of DDX21 DEV mutation in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 DEV mutation

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUE1-TSP-ORF68 R443A

Plasmid

#162652

Purpose

Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid R443 mutated to alanine

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-3GFP-kanMX6

Plasmid

#105148

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3GFP-kanMX6

Use

Tags

3GFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab1a

Plasmid

#203687

Purpose

Expresses N8C_Fab1a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab1a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-myc-ASPM CTR

Plasmid

#27669

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Contains carboxy terminal region of ASPM (amino a…

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pCI-Neo-mdm2C438L/2854

Plasmid

#11496

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

C438L

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-mdm2C464A/2855

Plasmid

#11493

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C464A

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-3YFP-kanMX6

Plasmid

#105149

Purpose

Yeast gene targeting

Depositor

David Kovar, Thomas Pollard, Jian-Qiu Wu

Article

Wu et al J Cell Biol. 2006 Jul 31;174(3):391-402.

Insert

3YFP-kanMX6

Use

Tags

3YFP

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Use

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-GFP (F64L, S65T, V163A)-kanMX6

Plasmid

#53183

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

kanMX6

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Expression

Mutation

Promoter

Available since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pCI-Neo-mdm2H452A/2858

Plasmid

#11497

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

H452A

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Use

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJB128 (MatP-mEos2)

Plasmid

#72653

Purpose

Inducible expression of MatP-mEos2 in bacteria

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

MatP-mEos2

Use

Tags

mEos2

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28b(+)-DR RecR(34-220)

Plasmid

#12618

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Deinococcus radiodurans RecR(34-220)

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-33

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-mdm2C438L/2857

Plasmid

#11494

Depositor

Allan Weissman

Article

Fang et al J Biol Chem. 2000 Mar 24. 275(12):8945-5

Insert

mdm2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C438L

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pCW-LIC-N8C_Fab2b

Plasmid

#203692

Purpose

Expresses N8C_Fab2b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab2b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab5a

Plasmid

#203693

Purpose

Expresses N8C_Fab5a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab5a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab2a

Plasmid

#203688

Purpose

Expresses N8C_Fab2a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab2a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab1b

Plasmid

#203691

Purpose

Expresses N8C_Fab1b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab1b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge