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pcDNA3-FKBP-DMVF-181-C

Plasmid

#87611

Purpose

FKBP-tagged C-terminal Dronpa fragment for BiFC.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

FKBP-DMVF181-C

Tags

6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBCN155-MXMT

Plasmid

#79815

Purpose

CFP-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

CN155/CFP (1–155)

Expression

Plant

Promoter

CaMV35S and CaMV 35S promoter

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v2

Plasmid

#154894

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Publication

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERBB3 (ERBB3 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB2-v1

Plasmid

#154901

Purpose

Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Publication

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERBB2 (ERBB2 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCambia2300-Aar1-ccdB-GmCH

Plasmid

#254405

Purpose

Gateway-compatible destination vector for bifluorescence complementation (BiFC). The plasmid carries Gus-mCherry expressed from the Arabidopsis Ubiquitin 10 promoter.

Depositor

Maria Harrison

Publication

Ivanov et al Science. 2024 Jan 26;383(6681):443-448.

Type

Empty backbone

Expression

Plant

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-ITGB1-V1

Plasmid

#215454

Purpose

Expression vector with integrin beta1 tagged with the BiFC V1 fragment

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CMV

Available since

Nov. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Dok1-V2

Plasmid

#215452

Purpose

Expression vector with Dok1 tagged with the BiFC V2 fragment

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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JW776

Plasmid

#64817

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rCUC2

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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JW777

Plasmid

#64818

Purpose

BiFC CUC3

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

C-terminal domain Firefly

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIR197

Plasmid

#64819

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

C-terminal domain Firefly

Mutation

rCUC2

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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HLH Clock Pas 1-401 VenN L113E

Plasmid

#47356

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L113E

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN F122D

Plasmid

#47357

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, F122D

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN W284A

Plasmid

#47358

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, W284A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN V315R

Plasmid

#47359

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, V315R

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
HLH Clock Pas 1-401 VenN L57E/L113E/W284A

Plasmid

#47360

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L57E/L113E/W284A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-FRB-DMVF-181-N

Plasmid

#87609

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

Lyn-FRB-DMVF181-N

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LifeAct-FRB-DMVF-181-N

Plasmid

#87610

Purpose

FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

LifeAct-FRB-DMVF181-N

Tags

17-aa sequence from Abp140 (aka "Lifeact&quo…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBGN155-MXMT

Plasmid

#79817

Purpose

GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

GN155/GFP (1–155)

Expression

Plant

Promoter

CaMV35S and CaMV 35S promoter

Available since

Aug. 18, 2016

Availability

Academic Institutions and Nonprofits only

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HA-GFP1-10-actin-pcDNA4-TO

Plasmid

#118370

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

actin beta (ACTB Human)

Tags

HA-GFP1-10

Expression

Mammalian

Promoter

CMV/TO

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBRC169-DXMT

Plasmid

#79822

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaDXMT1

Use

Reporter plasmid

Tags

RC169/DsREDmonomer (169–225)

Expression

Plant

Promoter

CaMV35S and CaMV 35S promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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