We narrowed to 9,077 results for: mel

1. pGex4t2 with full-length SUUR

   Plasmid

   #112990

   Purpose

   Expresses fusion of GST and full-length Drosophila SUUR

   Depositor

   Terry Orr-Weaver

   Article

   Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

   Insert

   SuUR (SuUR Fly)
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

   Plasmid

   #196973

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GFP and GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

   Plasmid

   #196976

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST

   Depositor

   Lukas Kapitein

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GST and SNAP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Opie2-PuroR[dsxM]

   Plasmid

   #131616

   Purpose

   Expresses a functional PuroR only in males and dsRed in both genders.

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

   Inserts

   PuroR[dsxM] (dsx Fly)
   dsRed
   

   Expression

   Insect

   Promoter

   Opie2 and hr5Ie1

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAL4DBD-SuUR f.l

   Plasmid

   #112995

   Purpose

   P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

   Insert

   SuUR (SuUR Fly)
   

   Use

   P insertion vector

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. RP132 pUAST EGFP:Or43a

   Plasmid

   #59523

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Or43a

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Or43a (Or43a Fly)
   

   Tags

   EGFP

   Expression

   Insect

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. KHBD00610

   Plasmid

   #39687

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG31169 (CG43342 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

8. KHBD00621

   Plasmid

   #39693

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG31392 (CG42726 Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMesh1*

   Plasmid

   #175591

   Purpose

   Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Mesh1 (Mesh1 Fly)
   

   Tags

   Fused with a flexible GS linker to cerulean

   Expression

   Bacterial

   Mutation

   Codon optimized for translation in E.coli. AGGAGG…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTripz Casp1-IRES-mCherry

    Plasmid

    #187294

    Purpose

    This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

    Depositor

    Alpha Yap

    Article

    Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

    Insert

    Caspase1 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    CMV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis(TEV)-dDrp1-StrepII

    Plasmid

    #174421

    Purpose

    Expresses D. melanogaster Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1 (Drp1 Fly)
    

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hy_pMT Flag-Hel25E WT

    Plasmid

    #110123

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOSGE_DUM1H01

    Plasmid

    #71090

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    nej (nej Fly)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGex4t2 with 244-458AA SUUR fragment

    Plasmid

    #112989

    Purpose

    Expresses fusion of GST and amino acids 244-458 of Drosophila SUUR

    Depositor

    Terry Orr-Weaver

    Article

    Nordman et al Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10

    Insert

    SuUR (SuUR Fly)
    

    Expression

    Bacterial

    Mutation

    amino acids 244-458

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. KHBD00717

    Plasmid

    #39745

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG12124 (mxc Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

17. KHBD00327

    Plasmid

    #34450

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG18783 (Kr-h1 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P-1 BICC FL

    Plasmid

    #112998

    Purpose

    For protein expression and purification of full-length Drosophila BicC

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length BicC (BicC Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-white[coffee]

    Plasmid

    #84006

    Purpose

    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4

    Depositor

    Phillip Zamore

    Article

    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

    Insert

    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    

    Use

    Unspecified

    Mutation

    A GC-to-AA mutation that creates a G589E missense…

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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