We narrowed to 1,129 results for: ncl

1. pET303-colE1T

   Plasmid

   #155180

   Purpose

   Expresses colicin E1 residues 1-190

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-190
   

   Use

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-190

   Promoter

   T7

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBH559_Tet-HisCas14a1Locus

   Plasmid

   #112502

   Purpose

   Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. p102_gRNA_Sa_mClover

   Plasmid

   #213778

   Purpose

   Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sa gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p103_gRNA_Sp_mClover

   Plasmid

   #213779

   Purpose

   Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sp gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWALIUM-intron 3x UAS attB

   Plasmid

   #206360

   Purpose

   includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

   Depositor

   Erdem Bangi

   Article

   Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ac5-STABLE1-blast

   Plasmid

   #208033

   Purpose

   Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS_KS_attB2_SA(1)_T2ALexA

   Plasmid

   #125221

   Purpose

   Artificial phase 1 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   LexA
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3E-APEX2-P2A-EGFP

   Plasmid

   #108896

   Purpose

   Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   APEX2-P2A-EGFP
   

   Use

   Multisite gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS_KS_attB2_SA(0)_T2ALexA

   Plasmid

   #125220

   Purpose

   Artificial phase 0 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

   Depositor

   Herman Dierick

   Article

   Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

   Insert

   LexA
   

   Use

   Other

   Tags

   T2A

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS_KS_attB2_SA(2)_T2ALexA

    Plasmid

    #125222

    Purpose

    Artificial phase 2 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

    Depositor

    Herman Dierick

    Article

    Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

    Insert

    LexA
    

    Use

    Other

    Tags

    T2A

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLBH545_Tet-Cas14a1_Locus

    Plasmid

    #112501

    Purpose

    Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14a1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTSA-13

    Plasmid

    #17327

    Depositor

    Takeshi Sano

    Article

    Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

    Insert

    streptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Truncated to include only amino acids 16-133 of m…

    Promoter

    Available since

    Feb. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. RGG-BcLOV-mCh

    Plasmid

    #208276

    Purpose

    Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    RGG-BcLOV-mCh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Fus-BcLOV-mCh

    Plasmid

    #208277

    Purpose

    Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXF276

    Plasmid

    #139048

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    lov domain (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSDH52

    Plasmid

    #139049

    Purpose

    MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cdk9-Halo donor

    Plasmid

    #113113

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cdk9 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PRSX8 eArchT3.0-EYFP

    Plasmid

    #89538

    Purpose

    Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

    Depositor

    Ruth Stornetta

    Article

    Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

    Insert

    eArchT 3.0
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    PRSx8

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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