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We narrowed to 12,258 results for: nsf

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Showing: 541 - 560 of 12258 results

  1. TMD24

    Plasmid
    #163678
    Purpose
    Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163678-plasmid-map-sequence-id-322683

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  2. FKBP-ST*-GFP

    Plasmid
    #163669
    Purpose
    Expresses FKBP tagged partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Tags
    FK506 binding protein (FKBP) and GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163669-plasmid-map-sequence-id-322815

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  5. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    T. J. Kappock
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available Since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72427-plasmid-map-sequence-id-137744

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  9. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  11. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

    Plasmid
    #192835
    Purpose
    Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells
    Depositor
    Karin Reinisch
    Article
    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070
    Insert
    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    Tags
    3xFLAG-PreScission cleavage site and 6xhis
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192835-plasmid-map-sequence-id-379957

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  12. pCSmEn2n4ERT(#234)

    Plasmid
    #184082
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(n4)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184082-plasmid-map-sequence-id-365718

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  13. pCSmEn2SRERT(#231)

    Plasmid
    #184080
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(SR)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …
    Promoter
    sCMV+SP6
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184080-plasmid-map-sequence-id-365710

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  14. pCSmEn25EERT(#232)

    Plasmid
    #184081
    Purpose
    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Rampon et al Development. 2015 May 15;142(10):1840-9.
    Insert
    6his-myc-chkEn2(5E)-ERT2
    Use
    Synthetic Biology
    Tags
    6his-Myc
    Expression
    Mammalian
    Mutation
    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …
    Promoter
    sCMV+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184081-plasmid-map-sequence-id-363924

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  16. pCMV-dCas13-inactive M3M14nes

    Plasmid
    #157855
    Purpose
    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant
    Depositor
    David Liu
    Article
    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103
    Insert
    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    Expression
    Mammalian
    Mutation
    PspCas13b Δ984-1090 H133A; METTL3 D395A
    Available Since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    157855-plasmid-map-sequence-id-307628

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  18. SSN-GFP

    Plasmid
    #163663
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163663-plasmid-map-sequence-id-322796

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  19. NSN-GFP

    Plasmid
    #163665
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF, TMD from ST6GAL1 and luminal domain from TNF in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from TNF…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163665-plasmid-map-sequence-id-322798

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  20. SNS-GFP

    Plasmid
    #163662
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, TMD from TNF and luminal domain from ST6GAL1 in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163662-plasmid-map-sequence-id-322795

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Showing: 541 - 560 of 12258 results