We narrowed to 12,952 results for: sequence

1. pUBC(k)-AT5G42080-mKOk

   Plasmid

   #224852

   Purpose

   Plasmid for expression of AT5G40280.1 coding sequence tagged with mKOk in plants

   Depositor

   Rebecca Roston

   Article

   LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

   Insert

   AT5G40280.1 (ERA1 Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. nCaMK2rep-S603A

   Plasmid

   #239625

   Purpose

   Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Changed serine 603 to Alanine

   Promoter

   hSyn

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXYB156

   Plasmid

   #239632

   Purpose

   Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)

   Depositor

   Xiaohan Yang

   Article

   Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

   Inserts

   sfGFPn_Y66-P1_loop_01-stem01-gRNA15
   gRNA16-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXYB085

   Plasmid

   #239637

   Purpose

   Plant RNA Vision constructs with 164 bp gRNAs together with RFP input

   Depositor

   Xiaohan Yang

   Article

   Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

   Inserts

   sfGFPn_Y66-P1_loop_01-stem01-gRNA09
   gRNA10-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXYB147

   Plasmid

   #239629

   Purpose

   Plant RNA Vision constructs with with 82 bp gRNAs targeting AT3G20840

   Depositor

   Xiaohan Yang

   Article

   Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

   Inserts

   sfGFPn_Y66-P1_loop_01-stem01-gRNA05
   gRNA06-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXYB084

   Plasmid

   #239636

   Purpose

   Plant RNA Vision constructs with 123 bp gRNAs together with RFP input

   Depositor

   Xiaohan Yang

   Article

   Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

   Inserts

   sfGFPn_Y66-P1_loop_01-stem01-gRNA07
   gRNA08-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXYB086

   Plasmid

   #239638

   Purpose

   Plant RNA Vision constructs with 325 bp gRNAs together with RFP input

   Depositor

   Xiaohan Yang

   Article

   Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

   Inserts

   sfGFPn_Y66-P1_loop_01-stem01-gRNA11
   gRNA12-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXYB155

   Plasmid

   #239631

   Purpose

   Plant RNA Vision constructs with with 82 bp gRNAs targeting Tobacco rattle virus (TRV)

   Depositor

   Xiaohan Yang

   Article

   Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

   Inserts

   sfGFPn_Y66-P1_loop_01-stem01-gRNA13
   gRNA14-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. MCUaaaa-Flag-pLYS1

   Plasmid

   #236785

   Purpose

   MCU tetrameric fusion. Only the initial MCU has a mitochondrial targeting sequence.

   Depositor

   Dipayan Chaudhuri

   Article

   Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

   Insert

   MCU tetramer (MCU Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJC2-TK2-3xFLAG

    Plasmid

    #217427

    Purpose

    Lentiviral overexpression of human TK2

    Depositor

    Jason Cantor

    Article

    Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

    Insert

    TK2 (TK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Insert sequence is a codon-optimized gBLOCK (IDT)

    Promoter

    CMV

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR1A 2xHA HCAR3

    Plasmid

    #226807

    Purpose

    Gateway entry vector for 2xHA HCAR3

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    HCAR3 (HCAR3 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Expression

    Mutation

    Sequence optimized from NP_006009.2

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMT-mMyh7CDS

    Plasmid

    #192849

    Purpose

    Plasmid to produce ISH probe for mMyh7 gene with CDS sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 7 (Myh7 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMT-mMyh7UTR

    Plasmid

    #192850

    Purpose

    Plasmid to produce ISH probe for mMyh7 gene with UTR sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 7 (Myh7 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57-CMV-MAAP6

    Plasmid

    #218245

    Purpose

    1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 6 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57-CMV-MAAP-SL08V9

    Plasmid

    #218251

    Purpose

    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. px552-sg-gria1-HT

    Plasmid

    #187652

    Purpose

    Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Inserts

    HaloTag donor sequence
    miRFP670
    

    Use

    AAV and CRISPR

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57-CMV-MAAP-SL01

    Plasmid

    #218248

    Purpose

    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCW57-CMV-MAAP9

    Plasmid

    #218246

    Purpose

    1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.

    Depositor

    David Schaffer

    Article

    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

    Insert

    Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-dCas9-FCPF

    Plasmid

    #220133

    Purpose

    Expresses dCas9-FCPF in E.coli

    Depositor

    Xinlai Cheng

    Article

    Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

    Insert

    dCas9-FCPF (cas9 S. pyogenes, Synthetic)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    FCPF sequence inserted on C-terminal

    Promoter

    T7

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGEM-601[insert(+1) TA-rich]

    Plasmid

    #221196

    Purpose

    601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    601[insert(+1)TA-rich]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    601 has an additional mutation added 22 bp from d…

    Promoter

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMAT_M13_6xSAG

    Plasmid

    #197096

    Purpose

    This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    Control peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET23a-H6-TEV-AncSZ

    Plasmid

    #214235

    Purpose

    Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

    Depositor

    Neel Shah

    Article

    Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

    Insert

    Syk kinase (SYK Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence gained through ancestral reconstruction …

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid

    #213401

    Purpose

    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

    Insert

    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pSC-cUnaG-LPETG

    Plasmid

    #207635

    Purpose

    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

    Depositor

    Cole DeForest

    Article

    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

    Insert

    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Amber stop codon at SC's critical lysine

    Promoter

    T7

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcS-RDG-C1C2

    Plasmid

    #200163

    Purpose

    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

    Depositor

    Masako Harada

    Article

    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

    Inserts

    RDG-C1C2
    C1C2 domain of lactadherin
    

    Use

    Tags

    HA and HIS

    Expression

    Mammalian

    Mutation

    sequence adopted from doi: 10.1371/journal.pone.0…

    Promoter

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pL0-EF-54730

    Plasmid

    #202163

    Purpose

    Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Sept. 6, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pL0_AC-27877

    Plasmid

    #202110

    Purpose

    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    27877 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove sapI sites fo…

    Promoter

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pL0_AC-Fld

    Plasmid

    #202125

    Purpose

    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    Fld promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pL0_AC-54730

    Plasmid

    #202109

    Purpose

    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    54730 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter sequence mutated to remove bsaI site for…

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge