We narrowed to 10,611 results for: t7

1. pJK227

   Plasmid

   #71126

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Tags

   GyrA intein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 RRN3

   Plasmid

   #67112

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RRN3 (RRN3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCellFree_G03 TCEB3B

   Plasmid

   #67117

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TCEB3B (ELOA2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCellFree_G03 ZFYVE9

   Plasmid

   #67134

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ZFYVE9 (ZFYVE9 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   F1359I

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDEST14-SARA

   Plasmid

   #67389

   Purpose

   Expression of native hSara in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pfMDH_K381A

   Plasmid

   #67732

   Purpose

   Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed lysine 381 to alanine

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pfMDH_N300D

   Plasmid

   #67728

   Purpose

   Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 300 to aspartate

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pfMDH_R373A

   Plasmid

   #67731

   Purpose

   Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed arginine 373 to alanine

   Promoter

   T7

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pfMDH_N300S

   Plasmid

   #67729

   Purpose

   Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed asparagine 300 to serine

   Promoter

   T7

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET11+PCNA3-CYP119

    Plasmid

    #66136

    Purpose

    Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11+PCNA3-CYP119(T214V)

    Plasmid

    #66137

    Purpose

    Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Tags

    Sulfolobus solfataricus PCNA3

    Expression

    Bacterial

    Mutation

    T214V

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b+PCNA3 T180C

    Plasmid

    #66181

    Purpose

    Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    T180C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b+PCNA3 R112C

    Plasmid

    #66180

    Purpose

    Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b+PCNA2 E105C

    Plasmid

    #66176

    Purpose

    Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

    Insert

    DNA polymerase sliding clamp 2
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E105C

    Promoter

    T7 promoter

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJexpress414_OmpA171_WTnoGirdles

    Plasmid

    #62653

    Purpose

    Wild-type OmpA with aromatic girdle residues replaced by leucine

    Depositor

    Timothy Whitehead

    Article

    Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

    Insert

    OmpA_noGirdles
    

    Use

    Synthetic Biology

    Tags

    6His and FlAsH

    Expression

    Bacterial

    Mutation

    Aromatic girdle residues mutated to Leu (W7, W15,…

    Promoter

    T7

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AI-CN491

    Plasmid

    #62594

    Purpose

    TALEN array receiving vector with half-site NN RVD

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Type

    Empty backbone

    Use

    TALEN; T7

    Tags

    3× FLAG and FOKI WT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AI-CN443

    Plasmid

    #62593

    Purpose

    TALEN array receiving vector with half-site NG RVD

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Type

    Empty backbone

    Use

    TALEN; T7

    Tags

    3× FLAG and FOKI WT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AI-CN442

    Plasmid

    #62591

    Purpose

    TALEN array receiving vector with half-site HD RVD

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Type

    Empty backbone

    Use

    TALEN; T7

    Tags

    3× FLAG and FOKI WT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AI-CN441

    Plasmid

    #62590

    Purpose

    TALEN array receiving vector with half-site NI RVD

    Depositor

    Joshua Grimley

    Article

    Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

    Type

    Empty backbone

    Use

    TALEN; T7

    Tags

    3× FLAG and FOKI WT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAD06 K. lactis telomerase RNA hairpin

    Plasmid

    #61895

    Purpose

    Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin (nt 229-273)
    

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only