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pHelper-T7IS1

Plasmid

#140631

Purpose

Expresses ShCAST under the T7 promoter and an empty sgRNA

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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S12.2_Bridge + Target

Plasmid

#127671

Purpose

Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

Depositor

Jennifer Phillips-Cremins

Publication

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Tags

HA

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

Plasmid

#126477

Purpose

Expresses Cas9-5xFlag-TdT in mammalian cells.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
5xFlag
TdT (DNTT Human)

Expression

Mammalian

Mutation

Changed codons to reduce repetitive sequences.

Promoter

CMV

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL210

Plasmid

#126451

Purpose

Expresses Cas9-XTEN-TdT in mammalian cells.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-XTEN-TdT (DNTT Streptococcus pyogenes, Human)

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-GCaMP6f

Plasmid

#102994

Purpose

Expresses histone fused GCaMP6f under the synapsin promoter

Depositor

David Cox

Publication

Skocek et al Nat Methods. 2018 Jun;15(6):429-432. doi

Insert

H2B-GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

human synapsin I promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pST1374-dCas9-GCN4

Plasmid

#113026

Purpose

Expresses dCas9-GCN4 in mammalian cells

Depositor

Xingxu Huang

Publication

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-GCN4 (GCN4 Budding Yeast, S. pyogenes)

Expression

Mammalian

Mutation

D10A, H840A, N863A

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pOD2047-bwmControl

Plasmid

#100006

Purpose

MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).

Depositor

Karen Oegema

Publication

Wang et al Development. 2017 Jun 15. pii: dev.15009

Inserts

zif-1 (zif-1 Nematode)
unc-119

Use

Targeting vector for mos1 transposon mediated sin…

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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L-CRISPR-CTN (MLL-ENL)

Plasmid

#69212

Purpose

Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

Depositor

Dirk Heckl

Publication

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDsred monomer C1- MLPH

Plasmid

#89236

Purpose

Expression of Dsred-tagged human Melanophilin in mammalian cells

Depositor

William Gahl

Publication

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human Melanophilin (MLPH Human)

Tags

Dsred monomer

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PML-ER-pBabe

Plasmid

#73556

Purpose

vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

ER

Expression

Mammalian

Promoter

SV40

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)

Plasmid

#70210

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Publication

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

deGFP

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSpp1-is2

Plasmid

#58248

Purpose

Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Publication

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Spp1-is2 (Spp1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-cxcr4b whim-gfp

Plasmid

#41089

Depositor

Anna Huttenlocher

Publication

Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

Insert

CXCR4b-WHIM (cxcr4b Zebrafish)

Tags

GFP

Expression

Bacterial

Mutation

deleted last 19 amino acids of cxcr4b

Promoter

mpx

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Thio6-His6-TEV-TARDBP(Δ1-273)

Plasmid

#260633

Purpose

Expresses TDP-43(Δ1-273) in bacterial strains

Depositor

Jennifer Lee

Publication

Watson et al J Am Chem Soc. 2026 Jun 17;148(23):23494

Insert

Transactive response DNA binding protein 43 (TARDBP Human)

Tags

thio6 expression sequence, hexahistidine, TEV pro…

Expression

Bacterial

Mutation

deleted amino acids 1-273

Available since

Sept. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pSCT375: pDonor(ZooTsy)_excision-reporter

Plasmid

#200873

Purpose

Reporter for ZooTsy excision.

Depositor

Feng Zhang

Publication

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAM256

Plasmid

#226900

Purpose

mEOS3.2-GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Publication

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

GS-SUMO-1(3-103)-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB511-TP-UnaG-FLAG

Plasmid

#203479

Purpose

For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG-FLAG

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB560-NADK2

Plasmid

#203760

Purpose

For Agrobacterium transformation. tagRFP fused Arabidopsis thaliana NAD KINASE2 (NADK2) under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Publication

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

NAD KINASE2

Expression

Bacterial and Plant

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3_ReBphP-PCM_IRES_sfGFP

Plasmid

#166917

Purpose

Express ReBphP-PCM and sfGFP

Depositor

Andre Stiel

Publication

Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

Inserts

ReBphP-PCM
sfGFP

Expression

Mammalian

Promoter

CMV

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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