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pQlinkH-PTPIP51(236-470)

Plasmid

#170530

Purpose

Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700]-Ss(424)

Plasmid

#166852

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

pCu

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHES 946

Plasmid

#112644

Purpose

blue light inducible cryptochrome based optogenetic expression system

Depositor

Hana El-Samad

Article

Harrigan et al Cell. 2018 Oct 18;175(3):877-886.e10. do

Insert

RTG3AD-GSx7-CIB1-SV40NLS (CIB1 Budding Yeast, Mustard Weed, Synthetic)

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 Q147A, M151A, I154A

Plasmid

#74616

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Q147A, M151A, I154A

Promoter

T7

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(Y305P)

Plasmid

#69557

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

Codon TAT encoding tyrosine 305 was changed to CC…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b(282-470)

Plasmid

#52125

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(282-470) (Mapk8ip1 Mouse)

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-281 and 471-707

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pLG-Sty

Plasmid

#33149

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Tags

LacZ

Expression

Yeast

Mutation

Sty1 site upstream of intron was cut/filled/ligat…

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA E171Q HDEL

Plasmid

#32123

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

HDEL and Kar2

Expression

Yeast

Mutation

K108A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA K108A HDEL

Plasmid

#32121

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

HDEL and Kar2

Expression

Yeast

Mutation

K108A

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-Flag-Tipin-L195A

Plasmid

#23118

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed Leucine 195 to Alanine

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
His-13bCTS-GFP

Plasmid

#247243

Purpose

A bacterial expression plasmid encoding ciliary targeting sequence of mammalian Arl13b (amino acids 347-363) with N-terminal His and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

deleted amino acid 1-346,364-428

Promoter

T7 Promoter

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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Arl13b-RVEP-4A-GFP

Plasmid

#221161

Purpose

Expresses mammalian Arl13b with RVEP4A mutation and GFP-tagged

Depositor

Lei Lu

Article

Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi

Insert

Arl13B (ARL13B Human)

Tags

EGFP

Expression

Mammalian

Mutation

RVEP at 358-361 mutated to AAAA

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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PB-PE SpG_ATH51

Plasmid

#242072

Purpose

Expresses SpG_ATH51 prime editor and pegRNA in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpG_ATH51_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

bpSV40 NLS

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-WT

Plasmid

#242749

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TCTN3 minigene (pAVA3939)

Plasmid

#239345

Purpose

Expresses TCTN3 minigene (EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG)

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

EFS-6xMyc-TCTN3-Ex10-I10-Ex11-copGFP-3xFLAG-BGH polyA (TCTN3 Human)

Tags

3xFLAG, 6xMyc, and CopGFP

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_EXOSC8 (pP18(T)_B1-AVA4071)

Plasmid

#239306

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting EXOSC8

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting EXOSC8 (EXOSC8 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_SKIV2L2 (pAVA3251)

Plasmid

#239323

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNAs targeting SKIV2L2

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting SKIV2L2 (MTREX Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_2xsgRNAs_ZCRB1 (pAVA3300)

Plasmid

#239325

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and two sgRNA stargeting ZCRB1

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA1 and 7SK-driven sgRNA2 targeting ZCRB1 (ZCRB1 Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_C1D (pP18(T)_B9-AVA4070)

Plasmid

#239296

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting C1D

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

U6-driven sgRNA targeting C1D (C1D Human)

Use

CRISPR and Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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