We narrowed to 14,477 results for: NTS;

1. pDualEX-SMN2

   Plasmid

   #214431

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM9037

   Plasmid

   #153225

   Purpose

   Template for PCR amplification of the constant part of guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   guide RNA constant part, carotenoid marker
   

   Use

   Synthetic Biology

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM65881

   Plasmid

   #153215

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDualIN-SMN2

   Plasmid

   #214430

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUSE-Src-YF-UniRapR-mCerulean-myc

   Plasmid

   #45381

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   Src-YF-UniRapR-mCerulean-myc
   

   Tags

   Myc, Myr, and mCerulean

   Expression

   Mammalian

   Mutation

   changed mSrc Tyrosine 529 to Phenylalanine

   Promoter

   CMV

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDR111_mannose_Bgal NLS

   Plasmid

   #188398

   Purpose

   Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Promoter

   D-mannose

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SlugABE-NNG

   Plasmid

   #214356

   Purpose

   Expresses SlugABE-NNG

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugABE-NNG
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Q782R/S888R/L906R/N984S/E1012K/K1016I

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mH2A1.1-CT-MYC

   Plasmid

   #45166

   Depositor

   Brian Chadwick, Hunt Willard

   Article

   Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

   Insert

   H2Afy (MACROH2A1 Human)
   

   Tags

   HIS and MYC

   Expression

   Mammalian

   Available Since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Pb-TRE3G-SpCas9-IRES-blast

    Plasmid

    #195506

    Purpose

    Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

    Depositor

    Joanna Wysocka

    Article

    Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

    Insert

    spCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mH2A1.2-CT-MYC

    Plasmid

    #45168

    Depositor

    Brian Chadwick, Hunt Willard

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    HIS and MYC

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3 IKKe flag

    Plasmid

    #26201

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. MDH1-PGK-FLAG-TAT-86-E

    Plasmid

    #35978

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    transactivating protein (tat Human Immunodeficiency Virus)
    

    Use

    RNAi and Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    From the "E" HIV clade

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDESTpK7WG2mito

    Plasmid

    #158726

    Purpose

    Plant mitoTALEN vector assembly, Destination vector

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    mitochondrial presequence in front of attR1 in pk7WG2
    

    Expression

    Plant

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-E-Syt3

    Plasmid

    #66832

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt3 (ESYT3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoG

    Plasmid

    #238235

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoG
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5_mCherry-P300

    Plasmid

    #238258

    Purpose

    For overexpression of mCherry-P300

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P300
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2/CMV-Luc/PGK-SB100X

    Plasmid

    #192869

    Purpose

    Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

    Depositor

    Maria Castro, David Largaespada

    Article

    Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

    Inserts

    luc
    SB100X
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-E-Syt1

    Plasmid

    #66830

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt1 (ESYT1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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