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We narrowed to 9,921 results for: pET

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Showing: 5661 - 5680 of 9921 results

  1. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Deborah Leckband
    Publication
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71711-plasmid-map-sequence-id-142367

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  3. SETD1A

    Plasmid
    #32868
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETD1A (SETD1A Human)
    Tags
    His tag (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  4. TBC1D7

    Plasmid
    #32047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBC1D7 (TBC1D7 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  5. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Publication
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  7. pcDNA3-ER-CaNAR2

    Plasmid
    #64732
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNAR2
    Tags
    Cerulean3, N-terminal 27 amino acids from CYP450,…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    64732-plasmid-map-sequence-id-109351

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  8. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  9. NRASA

    Plasmid
    #25256
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NRASA:M1-N172 (NRAS Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M1-N172
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    25256-plasmid-map-sequence-id-499692

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  10. pThermeleon

    Plasmid
    #86120
    Purpose
    Bandpass temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Publication
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…
    Promoter
    pR/pL, pR/pL, pLacI, and pTlpA
    Available since
    March 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86120-plasmid-map-sequence-id-173201

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Showing: 5661 - 5680 of 9921 results