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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,590 results for: sequence

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Showing: 5661 - 5680 of 13590 results

  1. CBLC

    Plasmid
    #39078
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBLC (CBLC Human)
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    A64E
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. pKG326

    Plasmid
    #69753
    Purpose
    Retroviral expression of CENP-T aa 1-531
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-531 (CENPT Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    E.coli optimized sequence; A216T and G459R mutati…
    Available Since
    Dec. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pCAGGS-△MTS-TFAM-V5

    Plasmid
    #200934
    Purpose
    expresses △MTS-TFAM-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    Transcription Factor A, Mitochondrial (TFAM Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    deleted mitochondrial targeting sequence (MTS)
    Promoter
    CAG
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200934-plasmid-map-sequence-id-410627

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  7. Venus-30aa-Noxa-4E-pEGFP-C1

    Plasmid
    #177424
    Purpose
    To express Venus fused to Noxa-4E, with a longer linker sequence between compared to Addgene #166745.
    Depositor
    David Andrews
    Insert
    Noxa, PMA-induced protein 1 (PMAIP1 Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    177424-plasmid-map-sequence-id-348448

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  8. pMX-Flag-GFP_ENPP5

    Plasmid
    #227956
    Purpose
    Control plasmid. Important note: Construct with N-terminally tagged GFP which might mask the signal sequence for proper localization of the protein
    Depositor
    Alessandro Ori
    Insert
    ENPP5 (ENPP5 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Available Since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    227956-plasmid-map-sequence-id-461327

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  9. pDual_dsCas9_Venus_hs_NRF2

    Plasmid
    #214692
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human NRF2
    Depositor
    Kevin Janes
    Insert
    dgRNA_NRF2 (NFE2L2 Human)
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214692-plasmid-map-sequence-id-428724

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  10. pAc5.1B-lambdaN-HA-DmBgcn_AA

    Plasmid
    #148207
    Purpose
    Insect Expression of DmBgcn
    Depositor
    Elisa Izaurralde
    Insert
    DmBgcn (bgcn Fly)
    Expression
    Insect
    Mutation
    19 silent mutations compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148207-plasmid-map-sequence-id-290655

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  11. pcDNA3.1-MS2-HA-HshnRNPM_243-615_Z

    Plasmid
    #148127
    Purpose
    Mammalian Expression of HshnRNPM_243-615
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPM_243-615 (HNRNPM Human)
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence of t…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148127-plasmid-map-sequence-id-290574

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  12. pT7-EGFP-C1-HsNot1iso1_1085-1605-del1_V

    Plasmid
    #147750
    Purpose
    Mammalian Expression of HsNot1iso1_1085-1605-del1
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1_1085-1605-del1 (CNOT1 Human)
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147750-plasmid-map-sequence-id-290197

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  13. pT7-EGFP-C1-HsNot1iso1_1085-1605-del2_V

    Plasmid
    #147751
    Purpose
    Mammalian Expression of HsNot1iso1_1085-1605-del2
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1_1085-1605-del2 (CNOT1 Human)
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147751-plasmid-map-sequence-id-290198

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  14. pT7-EGFP-C1-HsNot1iso1_1085-1605-del3_V

    Plasmid
    #147752
    Purpose
    Mammalian Expression of HsNot1iso1_1085-1605-del3
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1_1085-1605-del3 (CNOT1 Human)
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147752-plasmid-map-sequence-id-290199

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  16. mCerulean3-BclXL-ActA-pEGFP-C1

    Plasmid
    #177412
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177412-plasmid-map-sequence-id-348192

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  17. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177414
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177414-plasmid-map-sequence-id-348199

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  18. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177419-plasmid-map-sequence-id-348305

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  19. mCerulean3-Bcl2-ActA-s2193

    Plasmid
    #177420
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    Human ferritin
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177420-plasmid-map-sequence-id-358832

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  20. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177422-plasmid-map-sequence-id-353685

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Showing: 5661 - 5680 of 13590 results