We narrowed to 23,735 results for: fus

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pCMV-FLAG-PGRMC-Hybrid3

Plasmid

#173471

Purpose

N-terminal region of PGRMC1 fused to the transmembrane and C-terminal region of PGRMC2

Depositor

Bill Tsai

Article

Chen et al Nat Commun. 2021 Oct 13;12(1):5991. doi:

Insert

PGRMC1/PGRMC2 fusion (PGRMC1, PGRMC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

Plasmid

#162965

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r

Plasmid

#162964

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r

Plasmid

#162957

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r

Plasmid

#162955

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

Plasmid

#162954

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide#2

Plasmid

#121994

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-opa1 guide

Plasmid

#121995

Purpose

An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

opa1 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4E2H.210

Plasmid

#155212

Purpose

Expresses 4E2H.210 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBz172_CsoS2_N1_GFP

Plasmid

#140852

Purpose

His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 1 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz175_CsoS2_N4_GFP

Plasmid

#140855

Purpose

His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 4 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz173_CsoS2_N2_GFP

Plasmid

#140853

Purpose

His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz174_CsoS2_N3_GFP

Plasmid

#140854

Purpose

His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 3 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz26_CsoS2_N1-N2_GFP

Plasmid

#140851

Purpose

His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeats 1-2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

Plasmid

#140223

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRS424-ysg(G:H)

Plasmid

#138257

Purpose

Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

GFP targeting sgRNAs G and H

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-hsg(G:H)

Plasmid

#138259

Purpose

Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

mCherry targeting sgRNAs G and H

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-Occludin

Plasmid

#136637

Purpose

Expression of Occludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

Occludin (OCLN Chicken)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludin

Plasmid

#136638

Purpose

Expression of HAOccludin in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludin (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-OccludinCT3

Plasmid

#136639

Purpose

Expression of OccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

OccludinCT3 (OCLN Chicken)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCB6-HAOccludinCT3

Plasmid

#136640

Purpose

Expression of HAOccludinCT3 in mammalian cells

Depositor

Maria Balda, Karl Matter

Article

Balda et al J Cell Biol. 1996 Aug;134(4):1031-49. do

Insert

HAOccludinCT3 (OCLN Chicken)

Tags

HA (MQDLPGNDNSTAG)

Expression

Mammalian

Mutation

Deletion of C-terminal cytoplasid domain

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-GPI-pA

Plasmid

#109542

Purpose

Multisite gateway vector for expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2-6Gly-sfGFP

Plasmid

#124065

Purpose

E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-sfGFP

Tags

sfGFP

Expression

Bacterial

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(F317D, I325A)

Plasmid

#122275

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317H)

Plasmid

#122269

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Histidine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325A)

Plasmid

#122274

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317D, I325A)

Plasmid

#122273

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAD-Atg17(I325A)

Plasmid

#122272

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317Q)

Plasmid

#122271

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glutamine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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