We narrowed to 32,652 results for: CMV

1. pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

   Plasmid

   #176270

   Purpose

   Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   TadA-TadA*(8e)-dCasMINI-V4
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104095

   Purpose

   Expresses TREAT-siRNA reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Tags

   24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

   Expression

   Mammalian

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

   Plasmid

   #104094

   Purpose

   Expresses TREAT reporter in mammalian cells

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   Renilla luciferase
   

   Tags

   12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

   Expression

   Mammalian

   Promoter

   TetCMV

   Available Since

   Jan. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176459

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

   Plasmid

   #113700

   Purpose

   Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176460

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. R620-M04-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP N501Y

   Plasmid

   #170190

   Purpose

   ammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   N501Y

   Promoter

   CMV51

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF

   Plasmid

   #236173

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   ZNF35(5-8)-ZNF35(5-8)-deImmunLink-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/ZNF35 sites

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF

   Plasmid

   #236172

   Purpose

   PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factor

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Inserts

   ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
   H2B (H2BC11 Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV and four ZNF35/ZNF250 sites

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF

    Plasmid

    #236174

    Purpose

    PiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Inserts

    ZNF35/IKZF1(2-4)-deimmunLink-NZF
    H2B (H2BC11 Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and four ZNF35/IKZF1 sites

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210734

    Purpose

    Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Frodo sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid

    #217797

    Purpose

    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid

    #210735

    Purpose

    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Inserts

    SaSp D10A Cas9
    Gollum sgCAG
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, PolyA signal

    Expression

    Mammalian

    Mutation

    D10A, N terminal Sa Cas9, C-terminal Sp Cas9

    Promoter

    H1 and eCMV

    Available Since

    Jan. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

    Plasmid

    #170202

    Purpose

    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S SARS-CoV-2)
    

    Tags

    His6

    Expression

    Mammalian

    Mutation

    K417T, E484K, N501Y

    Promoter

    CMV51

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

    Plasmid

    #170194

    Purpose

    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S SARS-CoV-2)
    

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Mutation

    E484K

    Promoter

    CMV51

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213029

    Purpose

    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid

    #213028

    Purpose

    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    

    Tags

    3xFlag (ORF2p)

    Expression

    Mammalian

    Mutation

    E43S D145N double mutant EN- endonuclease catalyt…

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104140

    Purpose

    Expresses TREAT-5'TOP reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid

    #210513

    Purpose

    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Inserts

    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV and TetO

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid

    #113698

    Purpose

    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Inserts

    SaCas9 gRNA Cassete
    GFP
    

    Use

    AAV and CRISPR

    Tags

    KASH

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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